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https://hdl.handle.net/2440/119408
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dc.contributor.author | Ryan, M.H. | - |
dc.contributor.author | Kaur, P. | - |
dc.contributor.author | Nazeri, N.K. | - |
dc.contributor.author | Clode, P.L. | - |
dc.contributor.author | Keeble-Gagnère, G. | - |
dc.contributor.author | Doolette, A.L. | - |
dc.contributor.author | Smernik, R.J. | - |
dc.contributor.author | Van Aken, O. | - |
dc.contributor.author | Nicol, D. | - |
dc.contributor.author | Maruyama, H. | - |
dc.contributor.author | Ezawa, T. | - |
dc.contributor.author | Lambers, H. | - |
dc.contributor.author | Millar, A.H. | - |
dc.contributor.author | Appels, R. | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | Plant, Cell and Environment, 2019; 42(6):1987-2002 | - |
dc.identifier.issn | 0140-7791 | - |
dc.identifier.issn | 1365-3040 | - |
dc.identifier.uri | http://hdl.handle.net/2440/119408 | - |
dc.description.abstract | Crops with improved uptake of fertilizer phosphorus (P) would reduce P losses and confer environmental benefits. We examined how P-sufficient 6-week-old soil-grown Trifolium subterraneum plants, and 2-week-old seedlings in solution culture, accumulated P in roots after inorganic P (Pi) addition. In contrast to our expectation that vacuoles would accumulate excess P, after 7 days, X-ray microanalysis showed that vacuolar [P] remained low (<12 mmol kg-1 ). However, in the plants after P addition, some cortex cells contained globular structures extraordinarily rich in P (often >3,000 mmol kg-1 ), potassium, magnesium, and sodium. Similar structures were evident in seedlings, both before and after P addition, with their [P] increasing threefold after P addition. Nuclear magnetic resonance (NMR) spectroscopy showed seedling roots accumulated Pi following P addition, and transmission electron microscopy (TEM) revealed large plastids. For seedlings, we demonstrated that roots differentially expressed genes after P addition using RNAseq mapped to the T. subterraneum reference genome assembly and transcriptome profiles. Among the most up-regulated genes after 4 hr was TSub_g9430.t1, which is similar to plastid envelope Pi transporters (PHT4;1, PHT4;4): expression of vacuolar Pi-transporter homologs did not change. We suggest that subcellular P accumulation in globular structures, which may include plastids, aids cytosolic Pi homeostasis under high-P availability. | - |
dc.description.statementofresponsibility | Megan H. Ryan, Parwinder Kaur, Nazanin K. Nazeri, Peta L. Clode, Gabriel Keeble‐Gagnère | - |
dc.language.iso | en | - |
dc.publisher | Wiley Online Library | - |
dc.rights | © 2019 John Wiley & Sons Ltd. | - |
dc.source.uri | http://dx.doi.org/10.1111/pce.13531 | - |
dc.subject | X-ray microanalysis | - |
dc.subject | nuclear magnetic resonance spectroscopy | - |
dc.subject | phosphorous efficient crops | - |
dc.subject | plastids | - |
dc.subject | roots | - |
dc.subject | vacuoles | - |
dc.title | Globular structures in roots accumulate phosphorus to extremely high concentrations following phosphorus addition | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1111/pce.13531 | - |
dc.relation.grant | http://purl.org/au-research/grants/arc/FT140100103 | - |
dc.relation.grant | http://purl.org/au-research/grants/arc/CE140100008 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Doolette, A.L. [0000-0002-7504-9920] | - |
dc.identifier.orcid | Smernik, R.J. [0000-0001-6033-5855] | - |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest 8 |
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