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|Title:||Characterisation of barley resistance to rhynchosporium on chromosome 6HS|
|Citation:||Theoretical and Applied Genetics, 2019; 132(4):1089-1107|
|Max Coulter, Bianca Büttner, Kerstin Hofmann, Micha Bayer, Luke Ramsay, Günther Schweizer, Robbie Waugh, Mark E. Looseley, Anna Avrova|
|Abstract:||Key Message: Major resistance gene to rhynchosporium, Rrs18, maps close to the telomere on the short arm of chromosome 6H in barley. Rhynchosporium or barley scald caused by a fungal pathogen Rhynchosporium commune is one of the most destructive and economically important diseases of barley in the world. Testing of Steptoe × Morex and CIho 3515 × Alexis doubled haploid populations has revealed a large effect QTL for resistance to R. commune close to the telomere on the short arm of chromosome 6H, present in both populations. Mapping markers flanking the QTL from both populations onto the 2017 Morex genome assembly revealed a rhynchosporium resistance locus independent of Rrs13 that we named Rrs18. The causal gene was fine mapped to an interval of 660 Kb using Steptoe × Morex backcross 1 S₂ and S₃ lines with molecular markers developed from Steptoe exome capture variant calling. Sequencing RNA from CIho 3515 and Alexis revealed that only 4 genes within the Rrs18 interval were transcribed in leaf tissue with a serine/threonine protein kinase being the most likely candidate for Rrs18.|
|Keywords:||Chromosomes, Plant; Ascomycota; Hordeum; Genetic Markers; Physical Chromosome Mapping; Crosses, Genetic; Plant Diseases; Polymorphism, Single Nucleotide; Genes, Plant; Quantitative Trait Loci; Molecular Sequence Annotation; Disease Resistance|
|Rights:||© Springer-Verlag GmbH Germany, part of Springer Nature 2018|
|Appears in Collections:||Agriculture, Food and Wine publications|
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