Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/120805
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dc.contributor.author | Moheimani, F. | - |
dc.contributor.author | Koops, J. | - |
dc.contributor.author | Williams, T. | - |
dc.contributor.author | Reid, A.T. | - |
dc.contributor.author | Hansbro, P.M. | - |
dc.contributor.author | Wark, P.A. | - |
dc.contributor.author | Knight, D.A. | - |
dc.date.issued | 2018 | - |
dc.identifier.citation | Respiratory Research, 2018; 19(1):145-1-145-16 | - |
dc.identifier.issn | 1465-9921 | - |
dc.identifier.issn | 1465-993X | - |
dc.identifier.uri | http://hdl.handle.net/2440/120805 | - |
dc.description.abstract | Background: Specific microRNAs (miRNAs) play essential roles in airway remodeling in asthma. Infection with influenza A virus (IAV) may also magnify pre-existing airway remodeling leading to asthma exacerbation. However, these events remain to be fully defined. We investigated the expression of miRNAs with diverse functions including proliferation (miR-20a), differentiation (miR-22) or innate/adaptive immune responses (miR-132) in primary bronchial epithelial cells (pBECs) of asthmatics following infection with the H1N1 strain of IAV. Methods: pBECs from subjects (n = 5) with severe asthma and non-asthmatics were cultured as submerged monolayers or at the air-liquid-interface (ALI) conditions and incubated with IAV H1N1 (MOI 5) for up to 24 h. Isolated miRNAs were subjected to Taqman miRNAs assays. We confirmed miRNA targets using a specific mimic and antagomir. Taqman mRNAs assays and immunoblotting were used to assess expression of target genes and proteins, respectively. Results: At baseline, these miRNAs were expressed at the same level in pBECs of asthmatics and non-asthmatics. After 24 h of infection, miR-22 expression increased significantly which was associated with the suppression of CD147 mRNA and HDAC4 mRNA and protein expression in pBECs from non-asthmatics, cultured in ALI. In contrast, miR-22 remained unchanged while CD147 expression increased and HDAC4 remained unaffected in cells from asthmatics. IAV H1N1 mediated increases in SP1 and c-Myc transcription factors may underpin the induction of CD147 in asthmatics. Conclusion: The different profile of miR-22 expression in differentiated epithelial cells from non-asthmatics may indicate a self-defense mechanism against aberrant epithelial responses through suppressing CD147 and HDAC4, which is compromised in epithelial cells of asthmatics. | - |
dc.description.statementofresponsibility | Fatemeh Moheimani, Jorinke Koops, Teresa Williams, Andrew T. Reid, Philip M. Hansbro, Peter A. Wark, and Darryl A. Knight | - |
dc.language.iso | en | - |
dc.publisher | Springer Nature | - |
dc.rights | © The Author(s). 2018 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. | - |
dc.source.uri | http://dx.doi.org/10.1186/s12931-018-0851-7 | - |
dc.subject | Severe asthma; epithelial cells; microRNA; influenza A virus; airway remodeling | - |
dc.title | Influenza A virus infection dysregulates the expression of microRNA-22 and its targets; CD147 and HDAC4, in epithelium of asthmatics | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1186/s12931-018-0851-7 | - |
dc.relation.grant | http://purl.org/au-research/grants/nhmrc/1064405 | - |
dc.relation.grant | http://purl.org/au-research/grants/nhmrc/1079187 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Moheimani, F. [0000-0003-1239-3887] | - |
Appears in Collections: | Aurora harvest 8 Medicine publications |
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hdl_120805.pdf | Published Version | 2.18 MB | Adobe PDF | View/Open |
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