Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/120961
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Type: Journal article
Title: Neuropeptide Y1 receptor in immune cells regulates inflammation and insulin resistance associated with diet-induced obesity
Author: Macia, L.
Yulyaningsih, E.
Pangon, L.
Nguyen, A.D.
Lin, S.
Shi, Y.C.
Zhang, L.
Bijker, M.
Grey, S.
Mackay, F.
Herzog, H.
Sainsbury, A.
Citation: Diabetes, 2012; 61(12):3228-3238
Publisher: American Diabetes Association
Issue Date: 2012
ISSN: 0012-1797
1939-327X
Statement of
Responsibility: 
Laurence Macia, Ernie Yulyaningsih, Laurent Pangon, Amy D. Nguyen, Shu Lin, Yan C. Shi, Lei Zhang, Martijn Bijker, Shane Grey, Fabienne Mackay, Herbert Herzog, and Amanda Sainsbury
Abstract: Recruitment of activated immune cells into white adipose tissue (WAT) is linked to the development of insulin resistance and obesity, but the mechanism behind this is unclear. Here, we demonstrate that Y1 receptor signaling in immune cells controls inflammation and insulin resistance in obesity. Selective deletion of Y1 receptors in the hematopoietic compartment of mice leads to insulin resistance and inflammation in WAT under high fat-fed conditions. This is accompanied by decreased mRNA expression of the anti-inflammatory marker adiponectin in WAT and an increase of the proinflammatory monocyte chemoattractant protein-1 (MCP-1). In vitro, activated Y1-deficient intraperitoneal macrophages display an increased inflammatory response, with exacerbated secretion of MCP-1 and tumor necrosis factor, whereas addition of neuropeptide Y to wild-type macrophages attenuates the release of these cytokines, this effect being blocked by Y1 but not Y2 receptor antagonism. Importantly, treatment of adipocytes with the supernatant of activated Y1-deficient macrophages causes insulin resistance, as demonstrated by decreased insulin-induced phosphorylation of the insulin receptor and Akt as well as decreased expression of insulin receptor substrate 1. Thus, Y1 signaling in hematopoietic-derived cells such as macrophages is critical for the control of inflammation and insulin resistance in obesity.
Keywords: inflammation; insulin resistance
Rights: © 2012 by the American Diabetes Association. Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. See http://creativecommons.org/licenses/by -nc-nd/3.0/ for details.
DOI: 10.2337/db12-0156
Grant ID: http://purl.org/au-research/grants/nhmrc/427661
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