Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/123258
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Type: Journal article
Title: UV irradiation of skin enhances glycolytic flux and reduces migration capabilities in bone marrow-differentiated dendritic cells
Author: McGonigle, T.A.
Keane, K.N.
Ghaly, S.
Carter, K.W.
Anderson, D.
Scott, N.M.
Goodridge, H.S.
Dwyer, A.
Greenland, E.
Pixley, F.J.
Newsholme, P.
Hart, P.H.
Citation: The American Journal of Pathology, 2017; 187(9):2046-2059
Publisher: Elsevier
Issue Date: 2017
ISSN: 0002-9440
1525-2191
Statement of
Responsibility: 
Terence A. McGonigle, Kevin N. Keane, y Simon Ghaly, Kim W. Carter, Denise Anderson, Naomi M. Scott, Helen S. Goodridge, Amy Dwyer, Eloise Greenland, Fiona J. Pixley, Philip Newsholme, and Prue H. Hart
Abstract: A systemic immunosuppression follows UV irradiation of the skin of humans and mice. In this study, dendritic cells (DCs) differentiating from the bone marrow (BM) of UV-irradiated mice had a reduced ability to migrate toward the chemokine (C-C motif) ligand 21. Fewer DCs also accumulated in the peritoneal cavity of UV-chimeric mice (ie, mice transplanted with BM from UV-irradiated mice) after injection of an inflammatory stimulus into that site. We hypothesized that different metabolic states underpin altered DC motility. Compared with DCs from the BM of nonirradiated mice, those from UV-irradiated mice produced more lactate, consumed more glucose, and had greater glycolytic flux in a bioenergetics stress test. Greater expression of 3-hydroxyanthranilate 3,4-dioxygenase was identified as a potential contributor to increased glycolysis. Inhibition of 3-hydroxyanthranilate 3,4-dioxygenase by 6-chloro-dl-tryptophan prevented both increased lactate production and reduced migration toward chemokine (C-C motif) ligand 21 by DCs differentiated from BM of UV-irradiated mice. UV-induced prostaglandin E2 has been implicated as an intermediary in the effects of UV radiation on BM cells. DCs differentiating from BM cells pulsed in vitro for 2 hours with dimethyl prostaglandin E2 were functionally similar to those from the BM of UV-irradiated mice. Reduced migration of DCs to lymph nodes associated with increased glycolytic flux may contribute to their reduced ability to initiate new immune responses in UV-irradiated mice.
Keywords: Dendritic Cells
Rights: Copyright © 2017 American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved.
RMID: 1000006527
DOI: 10.1016/j.ajpath.2017.06.003
Grant ID: http://purl.org/au-research/grants/nhmrc/572660
http://purl.org/au-research/grants/nhmrc/1067209
Appears in Collections:Medicine publications

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