Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/123430
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Full metadata record
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dc.contributor.author | Takiguchi, M. | - |
dc.contributor.author | Dow, L.E. | - |
dc.contributor.author | Prier, J.E. | - |
dc.contributor.author | Carmichael, C.L. | - |
dc.contributor.author | Kile, B.T. | - |
dc.contributor.author | Turner, S.J. | - |
dc.contributor.author | Lowe, S.W. | - |
dc.contributor.author | Huang, D.C.S. | - |
dc.contributor.author | Dickins, R.A. | - |
dc.contributor.editor | Labrecque, N. | - |
dc.date.issued | 2013 | - |
dc.identifier.citation | PLoS One, 2013; 8(1):e54009-1-e54009-10 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | http://hdl.handle.net/2440/123430 | - |
dc.description.abstract | The tetracycline (tet)-regulated expression system allows for the inducible overexpression of protein-coding genes, or inducible gene knockdown based on expression of short hairpin RNAs (shRNAs). The system is widely used in mice, however it requires robust expression of a tet transactivator protein (tTA or rtTA) in the cell type of interest. Here we used an in vivo tet-regulated fluorescent reporter approach to characterise inducible gene/shRNA expression across a range of hematopoietic cell types of several commonly used transgenic tet transactivator mouse strains. We find that even in strains where the tet transactivator is expressed from a nominally ubiquitous promoter, the efficiency of tet-regulated expression can be highly variable between hematopoietic lineages and between differentiation stages within a lineage. In some cases tet-regulated reporter expression differs markedly between cells within a discrete, immunophenotypically defined population, suggesting mosaic transactivator expression. A recently developed CAG-rtTA3 transgenic mouse displays intense and efficient reporter expression in most blood cell types, establishing this strain as a highly effective tool for probing hematopoietic development and disease. These findings have important implications for interpreting tet-regulated hematopoietic phenotypes in mice, and identify mouse strains that provide optimal tet-regulated expression in particular hematopoietic progenitor cell types and mature blood lineages. | - |
dc.description.statementofresponsibility | Megumi Takiguchi, Lukas E. Dow, Julia E. Prier, Catherine L. Carmichael, Benjamin T. Kile, Stephen J. Turner, Scott W. Lowe, David C.S. Huang, Ross A. Dickins | - |
dc.language.iso | en | - |
dc.publisher | Public Library of Science | - |
dc.rights | © 2013 Takiguchi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | - |
dc.source.uri | http://dx.doi.org/10.1371/journal.pone.0054009 | - |
dc.subject | Hematopoietic System | - |
dc.title | Variability of inducible expression across the hematopoietic system of tetracycline transactivator transgenic mice | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1371/journal.pone.0054009 | - |
dc.relation.grant | http://purl.org/au-research/grants/nhmrc/575535 | - |
dc.relation.grant | http://purl.org/au-research/grants/nhmrc/1024599 | - |
dc.relation.grant | http://purl.org/au-research/grants/nhmrc/509693 | - |
dc.relation.grant | ARC | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Kile, B.T. [0000-0002-8836-8947] | - |
Appears in Collections: | Aurora harvest 8 Medicine publications |
Files in This Item:
File | Description | Size | Format | |
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hdl_123430.pdf | Published Version | 2.4 MB | Adobe PDF | View/Open |
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