Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/123723
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dc.contributor.author | Du, F. | - |
dc.contributor.author | Gong, W. | - |
dc.contributor.author | Bosca, S. | - |
dc.contributor.author | Tucker, M.R. | - |
dc.contributor.author | Vaucheret, H. | - |
dc.contributor.author | Laux, T. | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | Plant Communications, 2020; 1(1):100002-1-100002-11 | - |
dc.identifier.issn | 2590-3462 | - |
dc.identifier.issn | 2590-3462 | - |
dc.identifier.uri | http://hdl.handle.net/2440/123723 | - |
dc.description.abstract | Pluripotent stem cells localized in proliferating growth centers, the meristems, are the origin of life-long organ formation and growth in higher plants. In the shoot apical meristem of Arabidopsis thaliana, the closely related ARGONAUTE proteins AGO1 and ZLL/AGO10 bind miR165/166 species to regulate mRNAs of HD-ZIP III transcription factors that are essential to maintaining stem cells. Several genetic studies showed that AGO1 and ZLL/AGO10 act redundantly to maintain stem cells. By contrast, the reported biochemical data suggested antagonistic functions: AGO1 utilizes miR165/166 to slice HD-ZIP III mRNAs, whereas ZLL/AGO10 promotes degradation of miR165/166 and thus stabilizes HD-ZIP III mRNAs. How these different functions are balanced in stem cell regulation has remained enigmatic. Here, we show that autorepression of AGO1 through miR168-mediated slicing of its own RNA is required to maintain the ability of AGO1 to suppress HD-ZIP III mRNAs. Increased AGO1 expression, either in the miR168a-2 mutant or by transgenic expression, inhibits this ability despite the presence of high levels of miR165/166, effectively uncoupling HD-ZIP III and miR165/166 expression. AGO1 activity can be restored, however, by increasing the levels of chaperones SQN and HSP90, which promote assembly of RNA-induced silencing complex (RISC). This suggests that cellular abundance of SQN and HSP chaperones limits AGO1-mediated RNA interference in shoot meristem stem cell regulation. Localized misexpression of AGO1 indicates that the cells surrounding the shoot meristem primordium play a crucial role in stem cell development. Taken together, our study provides a framework that reconciles biochemical and genetic data, showing that restriction of AGO1 levels by miR168-mediated autorepression is key to RISC homeostasis and the function of AGO1 in stem cell regulation. | - |
dc.description.statementofresponsibility | Fei Du, Wen Gong, Sonia Bosca, Matthew Tucker, Herve, Vaucheret, and Thomas Laux | - |
dc.language.iso | en | - |
dc.publisher | Elsevier | - |
dc.rights | © 2019 The Authors.This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). | - |
dc.source.uri | http://dx.doi.org/10.1016/j.xplc.2019.100002 | - |
dc.subject | Arabidopsis; ARGONAUTE1; ZWILLE (ARGONAUTE10); HD-ZIP III; stem cell; shoot meristem | - |
dc.title | Dose-dependent AGO1-mediated inhibition of the miRNA165/166 pathway modulates stem cell maintenance in Arabidopsis shoot apical meristem | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1016/j.xplc.2019.100002 | - |
dc.relation.grant | http://purl.org/au-research/grants/arc/FT140100780 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Tucker, M.R. [0000-0003-4661-6700] | - |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest 4 |
Files in This Item:
File | Description | Size | Format | |
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hdl_123723.pdf | Published version | 3.48 MB | Adobe PDF | View/Open |
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