Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/123765
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Cannabinoid receptor 2 modulates maturation of dendritic cells and their capacity to induce hapten-induced contact hypersensitivity |
Author: | Gaffal, E. Kemter, A.M. Scheu, S. Leite Dantas, R. Vogt, J. Baune, B. Tüting, T. Zimmer, A. Alferink, J. |
Citation: | International Journal of Molecular Sciences, 2020; 21(2):1-12 |
Publisher: | MDPI AG |
Issue Date: | 2020 |
ISSN: | 1422-0067 1422-0067 |
Statement of Responsibility: | Evelyn Gaffal, Andrea M. Kemter, Stefanie Scheu, Rafael Leite Dantas, Jens Vogt, Bernhard Baune, Thomas Tüting, Andreas Zimmer and Judith Alferink |
Abstract: | Contact hypersensitivity (CHS) is an established animal model for allergic contact dermatitis. Dendritic cells (DCs) play an important role in the sensitization phase of CHS by initiating T cell responses to topically applied haptens. The cannabinoid receptors 1 (CB1) and 2 (CB2) modulate DC functions and inflammatory skin responses, but their influence on the capacity of haptenized DCs to induce CHS is still unknown. We found lower CHS responses to 2,4-dinitro-1-fluorobenzene (DNFB) in wild type (WT) mice after adoptive transfer of haptenized Cnr2-/- and Cnr1-/-/Cnr2-/- bone marrow (BM) DCs as compared to transfer of WT DCs. In contrast, induction of CHS was not affected in WT recipients after transfer of Cnr1-/- DCs. In vitro stimulated Cnr2-/- DCs showed lower CCR7 and CXCR4 expression when compared to WT cells, while in vitro migration towards the chemokine ligands was not affected by CB2. Upregulation of MHC class II and co-stimulatory molecules was also reduced in Cnr2-/- DCs. This study demonstrates that CB2 modulates the maturation phenotype of DCs but not their chemotactic capacities in vitro. These findings and the fact that CHS responses mediated by Cnr2-/- DCs are reduced suggest that CB2 is a promising target for the treatment of inflammatory skin conditions. |
Keywords: | CB1 CB2 CCL19 CXCL12 MHC allergic contact dermatitis cannabinoid receptors dendritic cells hapten 2,4-dinitro-1-fluorobenzene migration skin inflammation |
Rights: | © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
DOI: | 10.3390/ijms21020475 |
Published version: | http://dx.doi.org/10.3390/ijms21020475 |
Appears in Collections: | Aurora harvest 4 Medicine publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
hdl_123765.pdf | Published version | 663.18 kB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.