Please use this identifier to cite or link to this item:
Scopus Web of Science® Altmetric
Type: Journal article
Title: Real-time in vivo imaging of regional lung function in a mouse model of cystic fibrosis on a laboratory X-ray source
Author: Murrie, R.P.
Werdiger, F.
Donnelley, M.
Lin, Y.W.
Carnibella, R.P.
Samarage, C.R.
Pinar, I.
Preissner, M.
Wang, J.
Li, J.
Morgan, K.S.
Parsons, D.W.
Dubsky, S.
Fouras, A.
Citation: Scientific Reports, 2020; 10(1):447-447
Publisher: Springer Nature
Issue Date: 2020
ISSN: 2045-2322
Statement of
Rhiannon P. Murrie, Freda Werdiger, Martin Donnelley, Yu-wei Lin, Richard P. Carnibella ... David W. Parsons ... et al.
Abstract: Most measures of lung health independently characterise either global lung function or regional lung structure. The ability to measure airflow and lung function regionally would provide a more specific and physiologically focused means by which to assess and track lung disease in both pre-clinical and clinical settings. One approach for achieving regional lung function measurement is via phase contrast X-ray imaging (PCXI), which has been shown to provide highly sensitive, high-resolution images of the lungs and airways in small animals. The detailed images provided by PCXI allow the application of four-dimensional X-ray velocimetry (4DxV) to track lung tissue motion and provide quantitative information on regional lung function. However, until recently synchrotron facilities were required to produce the highly coherent, high-flux X-rays that are required to achieve lung PCXI at a high enough frame rate to capture lung motion. This paper presents the first translation of 4DxV technology from a synchrotron facility into a laboratory setting by using a liquid-metal jet microfocus X-ray source. This source can provide the coherence required for PCXI and enough X-ray flux to image the dynamics of lung tissue motion during the respiratory cycle, which enables production of images compatible with 4DxV analysis. We demonstrate the measurements that can be captured in vivo in live mice using this technique, including regional airflow and tissue expansion. These measurements can inform physiological and biomedical research studies in small animals and assist in the development of new respiratory treatments.
Keywords: Lung
Cystic Fibrosis
Disease Models, Animal
Tomography, X-Ray Computed
Pulmonary Ventilation
Time Factors
Rights: © Te Author(s) 2020. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Te images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit
DOI: 10.1038/s41598-019-57376-w
Grant ID:
Appears in Collections:Aurora harvest 4
Medicine publications

Files in This Item:
File Description SizeFormat 
hdl_124216.pdfPublished version1.4 MBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.