Please use this identifier to cite or link to this item:
Scopus Web of Science® Altmetric
Type: Journal article
Title: The transgenerational effects of oocyte mitochondrial supplementation
Author: St John, J.
Makanji, Y.
Johnson, J.
Tsai, T.-S.
Lagondar, S.
Rodda, F.
Sun, X.
Pangestu, M.
Chen, P.
Temple-Smith, P.
Citation: Scientific Reports, 2019; 9(1):6694-1-6694-12
Publisher: Springer Nature
Issue Date: 2019
ISSN: 2045-2322
Statement of
Justin C. St. John, Yogeshwar Makanji, Jacqueline L. Johnson, Te-Sha Tsai, Simone Lagondar, Fleur Rodda, Xin Sun, Mulyoto Pangestu, Penny Chen, Peter Temple-Smith
Abstract: Many women suffer from either failed fertilisation or their embryos arrest early during development. Autologous mitochondrial supplementation has been proposed as an assisted reproductive technology to overcome these problems. However, its safety remains to be tested in an animal model to determine if there are transgenerational effects. We have supplemented oocytes with autologous populations of mitochondria to generate founders. We mated the female founders and their offspring to produce three generations. We assessed litter size, the ovarian reserve, and weight gain and conducted a full histopathological analysis from each of the three generations. Across the generations, we observed significant increases in litter size and in the number of primordial follicles in the ovary matched by changes in global gene expression patterns for these early-stage oocytes. However, full histopathological analysis revealed that cardiac structure was compromised in first and second generation offspring, which could seriously affect the health of the offspring. Furthermore, the offspring were prone to increased weight gain during early life. Mitochondrial supplementation appears to perturb the regulation of the chromosomal genome resulting in transgenerational phenotypic gains and losses. These data highlight the need for caution when using autologous mitochondrial supplementation to treat female factor infertility.
Keywords: Myocardium
Mice, Inbred C57BL
Animals, Newborn
Body Weight
Reproductive Techniques, Assisted
Sperm Injections, Intracytoplasmic
Embryo Implantation
Litter Size
Ovarian Reserve
Description: Published online: 30 April 2019
Rights: © The Author(s) 2019. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit
DOI: 10.1038/s41598-019-43135-4
Published version:
Appears in Collections:Aurora harvest 4
Paediatrics publications

Files in This Item:
File Description SizeFormat 
hdl_124537.pdfPublished version2.29 MBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.