Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/126421
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Salivary small extracellular vesicles associated miRNAs in periodontal status-a pilot study |
Author: | Han, P. Bartold, P.M. Salomon, C. Ivanovski, S. |
Citation: | International Journal of Molecular Sciences, 2020; 21(8):1-13 |
Publisher: | MDPI |
Issue Date: | 2020 |
ISSN: | 1422-0067 1422-0067 |
Statement of Responsibility: | Pingping Han, Peter Mark Bartold, Carlos Salomon and Saso Ivanovski |
Abstract: | This pilot study aims to investigate whether salivary small extracellular vesicle (sEV)-associated microRNAs could act as potential biomarkers for periodontal disease status. Twenty-nine participants (10 who were healthy, nine with gingivitis, 10 with stage III/IV periodontitis) were recruited and unstimulated whole saliva samples were collected. Salivary sEVs were isolated using the size-exclusion chromatography (SEC) method and characterised by morphology, EV-protein and size distribution using transmission electron microscopy (TEM), Western Blot and Nanoparticle Tracking Analysis (NTA), respectively. Ten mature microRNAs (miRNAs) in salivary sEVs and saliva were evaluated using RT-qPCR. The discriminatory power of miRNAs as biomarkers in gingivitis and periodontitis versus healthy controls was evaluated by Receiver Operating Characteristics (ROC) curves. Salivary sEVs were comparable to sEVs morphology, mode, size distribution and particle concentration in healthy, gingivitis and periodontitis patients. Compared to miRNAs in whole saliva, three significantly increased miRNAs (hsa-miR-140-5p, hsa-miR-146a-5p and hsa-miR-628-5p) were only detected in sEVs in periodontitis when compared to that of healthy controls, with a good discriminatory power (area under the curve (AUC) = 0.96) for periodontitis diagnosis. Our study demonstrated that salivary sEVs are a non-invasive source of miRNAs for periodontitis diagnosis. Three miRNAs that are selectively enriched in sEVs, but not whole saliva, could be potential biomarkers for periodontal disease status. |
Keywords: | biomarker discovery miRNAs periodontal disease salivary small extracellular vesicles size exclusion chromatography |
Rights: | © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
DOI: | 10.3390/ijms21082809 |
Grant ID: | http://purl.org/au-research/grants/nhmrc/1114013 |
Published version: | http://dx.doi.org/10.3390/ijms21082809 |
Appears in Collections: | Aurora harvest 8 Chemistry and Physics publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
hdl_126421.pdf | Published version | 3.75 MB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.