Please use this identifier to cite or link to this item:
Scopus Web of Science® Altmetric
Type: Journal article
Title: A genome assembly of the barley 'transformation reference' cultivar golden promise
Author: Schreiber, M.
Mascher, M.
Wright, J.
Padmarasu, S.
Himmelbach, A.
Heavens, D.
Milne, L.
Clavijo, B.J.
Stein, N.
Waugh, R.
Citation: G3: Genes, Genomes, Genetics, 2020; 10(6):1823-1827
Publisher: Genetics Society of America
Issue Date: 2020
ISSN: 2160-1836
Statement of
Miriam Schreiber, Martin Mascher, Jonathan Wright, Sudharasan Padmarasu, Axel Himmelbach ... Robbie Waugh ... et al.
Abstract: Barley (Hordeum vulgare) is one of the most important crops worldwide and is also considered a research model for the large-genome small grain temperate cereals. Despite genomic resources improving all the time, they are limited for the cv Golden Promise, the most efficient genotype for genetic transformation. We have developed a barley cv Golden Promise reference assembly integrating Illumina paired-end reads, long mate-pair reads, Dovetail Chicago in vitro proximity ligation libraries and chromosome conformation capture sequencing (Hi-C) libraries into a contiguous reference assembly. The assembled genome of 7 chromosomes and 4.13Gb in size, has a super-scaffold N50 after Chicago libraries of 4.14Mb and contains only 2.2% gaps. Using BUSCO (benchmarking universal single copy orthologous genes) as evaluation the genome assembly contains 95.2% of complete and single copy genes from the plant database. A high-quality Golden Promise reference assembly will be useful and utilized by the whole barley research community but will prove particularly useful for CRISPR-Cas9 experiments.
Keywords: Barley
Golden Promise
reference assembly
Rights: © 2020 Schreiber et al.This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
DOI: 10.1534/g3.119.401010
Appears in Collections:Agriculture, Food and Wine publications
Aurora harvest 8

Files in This Item:
There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.