Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/126551
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Type: Journal article
Title: A genome assembly of the barley 'transformation reference' cultivar golden promise
Author: Schreiber, M.
Mascher, M.
Wright, J.
Padmarasu, S.
Himmelbach, A.
Heavens, D.
Milne, L.
Clavijo, B.J.
Stein, N.
Waugh, R.
Citation: G3: Genes, Genomes, Genetics, 2020; 10(6):1823-1827
Publisher: Genetics Society of America
Issue Date: 2020
ISSN: 2160-1836
2160-1836
Statement of
Responsibility: 
Miriam Schreiber, Martin Mascher, Jonathan Wright, Sudharasan Padmarasu, Axel Himmelbach ... Robbie Waugh ... et al.
Abstract: Barley (Hordeum vulgare) is one of the most important crops worldwide and is also considered a research model for the large-genome small grain temperate cereals. Despite genomic resources improving all the time, they are limited for the cv Golden Promise, the most efficient genotype for genetic transformation. We have developed a barley cv Golden Promise reference assembly integrating Illumina paired-end reads, long mate-pair reads, Dovetail Chicago in vitro proximity ligation libraries and chromosome conformation capture sequencing (Hi-C) libraries into a contiguous reference assembly. The assembled genome of 7 chromosomes and 4.13Gb in size, has a super-scaffold N50 after Chicago libraries of 4.14Mb and contains only 2.2% gaps. Using BUSCO (benchmarking universal single copy orthologous genes) as evaluation the genome assembly contains 95.2% of complete and single copy genes from the plant database. A high-quality Golden Promise reference assembly will be useful and utilized by the whole barley research community but will prove particularly useful for CRISPR-Cas9 experiments.
Keywords: Barley
Golden Promise
reference assembly
Rights: © 2020 Schreiber et al.This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
DOI: 10.1534/g3.119.401010
Appears in Collections:Agriculture, Food and Wine publications
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