Please use this identifier to cite or link to this item:
Scopus Web of Science® Altmetric
Type: Journal article
Title: A novel image analysis methodology for online monitoring of nucleation and crystal growth during solid state phase transformations
Author: Wu, J.
Xia, D.
Van Den Berg, F.
Amigo, J.
Rades, T.
Yang, M.
Rantanen, J.
Citation: International Journal of Pharmaceutics, 2012; 433(1-2):60-70
Publisher: Elsevier
Issue Date: 2012
ISSN: 0378-5173
Statement of
Jian X.Wu, Dengning Xia, Fransvan den Berg, José Manuel Amigo, Thomas Rades, Mingshi Yang, Jukka Rantanen
Abstract: This study focuses on the development of an automated image analysis method to extract information on nucleation and crystal growth from polarized light micrographs. Using the developed image analysis method, four parameters related to nucleation and crystal growth could be extracted from the images. These parameters were crystalline count (applied as a measure of nucleation), percentage area coverage, average equivalent diameter and average crystalline area (three last parameters applied as a measure for crystal growth). The developed image analysis method was used to investigate two pharmaceutically relevant case studies: first, nitrendipine antisolvent crystallization, and second, recrystallization of amorphous piroxicam solid dispersion in an aqueous environment. In both case studies, an amorphous-to-crystalline phase transformation were observed, which were successfully monitored using real-time Raman spectroscopy. For the both case studies, the parameters related to crystallization kinetics estimated by image analysis were in close agreement with the parameters estimated by Raman spectroscopy. The developed image analysis method proved to be a valuable tool for quantitative monitoring of nucleation and crystal growth with an obvious potential for high throughput screening.
Keywords: Polarized light microscopy; image analysis; nucleation; crystal growth; solid dispersion; antisolvent crystallization
Rights: © 2012 Elsevier B.V. All rights reserved.
DOI: 10.1016/j.ijpharm.2012.04.074
Appears in Collections:Aurora harvest 8
Chemistry publications

Files in This Item:
There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.