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https://hdl.handle.net/2440/127
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Type: | Journal article |
Title: | Application of 16S rDNA-DGGE to examine the microbial ecology associated with a social wasp Vespula germanica |
Author: | Reeson, A. Jankovic-Karasoulos, T. Kasper, M. Rogers, S. Austin, A. |
Citation: | Insect Molecular Biology, 2003; 12(1):85-91 |
Publisher: | Blackwell Science Ltd |
Issue Date: | 2003 |
ISSN: | 0962-1075 1365-2583 |
Statement of Responsibility: | A. F. Reeson, T. Jankovic, M. L. Kasper, S. Rogers and A. D. Au |
Abstract: | Invertebrates host numerous bacteria, with interactions ranging from pathogenesis to symbiosis. While certain symbiotic relationships have been well studied, little is known about the dynamics of these bacterial communities. Denaturing gradient gel electrophoresis (DGGE) was used to examine the bacterial microflora associated with the eusocial wasp Vespula germanica. DGGE profiles of larval guts revealed a variable microflora, suggesting that V. germanica is not dependent on a particular suite of mutualists. The variation in profiles was not related to season, nest size or macrohabitat. Sequences corresponding to Lactococcus, Lactobacillus, a novel Leuconostoc and two Rickettsiella grylli strains were obtained. DGGE proved to be a useful technique for characterizing the wasp microflora. Given the importance of microbial communities to invertebrates, there is much to be gained from the application of such techniques. |
Keywords: | Animals Wasps Rickettsia Lactococcus Leuconostoc Lactobacillus DNA, Bacterial RNA, Ribosomal, 16S Electrophoresis, Polyacrylamide Gel DNA Fingerprinting Polymerase Chain Reaction Sequence Analysis, DNA Ecosystem South Australia |
Description: | The definitive version is available at www.blackwell-synergy.com |
DOI: | 10.1046/j.1365-2583.2003.00390.x |
Published version: | http://www.blackwell-synergy.com/links/doi/10.1046/j.1365-2583.2003.00390.x |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest 6 Environment Institute Leaders publications |
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