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Type: Journal article
Title: Arabidopsis annexin1 mediates the radical-activated plasma membrane Ca²⁺- and K⁺-permeable conductance in root cells
Other Titles: Arabidopsis annexin1 mediates the radical-activated plasma membrane Ca(2+)- and K(+)-permeable conductance in root cells
Author: Laohavisit, A.
Shang, Z.
Rubio, L.
Cuin, T.A.
Véry, A.-.A.
Wang, A.
Mortimer, J.C.
Macpherson, N.
Coxon, K.M.
Battey, N.H.
Brownlee, C.
Park, O.K.
Sentenac, H.
Shabala, S.
Webb, A.A.R.
Davies, J.M.
Citation: The Plant Cell, 2012; 24(4):1522-1533
Publisher: American Society of Plant Biologists
Issue Date: 2012
ISSN: 1040-4651
Statement of
Anuphon Laohavisit, Zhonglin Shang, Lourdes Rubio, Tracey A. Cuin, Anne-Aliénor Véry, Aihua Wang ... et al.
Abstract: Plant cell growth and stress signaling require Ca2+ influx through plasma membrane transport proteins that are regulated by reactive oxygen species. In root cell growth, adaptation to salinity stress, and stomatal closure, such proteins operate downstream of the plasma membrane NADPH oxidases that produce extracellular superoxide anion, a reactive oxygen species that is readily converted to extracellular hydrogen peroxide and hydroxyl radicals, OH_. In root cells, extracellular OH_ activates a plasma membrane Ca2+-permeable conductance that permits Ca2+ influx. In Arabidopsis thaliana, distribution of this conductance resembles that of annexin1 (ANN1). Annexins are membrane binding proteins that can form Ca2+-permeable conductances in vitro. Here, the Arabidopsis loss-of-function mutant for annexin1 (Atann1) was found to lack the root hair and epidermal OH_-activated Ca2+- and K+-permeable conductance. This manifests in both impaired root cell growth and ability to elevate root cell cytosolic free Ca2+ in response to OH_. An OH_-activated Ca2+ conductance is reconstituted by recombinant ANN1 in planar lipid bilayers. ANN1 therefore presents as a novel Ca2+-permeable transporter providing a molecular link between reactive oxygen species and cytosolic Ca2+ in plants.
Keywords: Cell Membrane; Protoplasts; Arabidopsis; Plant Epidermis; Plant Roots; Hydroxyl Radical; Potassium; Calcium; Lipid Bilayers; Annexin A1; Calcium Channels; Arabidopsis Proteins; Recombinant Proteins; Cell Membrane Permeability; Ion Channel Gating; Diffusion; Shaker Superfamily of Potassium Channels; Plant Cells
Rights: © 2012 American Society of Plant Biologists. All rights reserved.
RMID: 1000031416
DOI: 10.1105/tpc.112.097881
Appears in Collections:Biochemistry publications

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