Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/132647
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Impact of in vitro embryo culture and transfer on blood pressure regulation in the adolescent lamb |
Other Titles: | Impact of in vitro embryo culture and transfer on blood pressure regulation in early postnatal life |
Author: | Padhee, M. McMillen, C. Zhang, S. Armitage, J.A. Head, G.A. MacLaughlin, S.M. Kleemann, D.O. Walker, S.K. Morrison, J.L. |
Citation: | Journal of Developmental Origins of Health and Disease, 2021; 12(5):731-737 |
Publisher: | Cambridge University Press |
Issue Date: | 2021 |
ISSN: | 2040-1744 2040-1752 |
Statement of Responsibility: | Monalisa Padhee, I. Caroline McMillen, Song Zhang, Severence M. MacLaughlin, James A. Armitage, Geoffrey A. Head, Jack R. T. Darby, Jennifer M. Kelly, Skye R. Rudiger, David O. Kleemann, Simon K. Walker and Janna L. Morrison |
Abstract: | Nutrition during the periconceptional period influences postnatal cardiovascular health. We determined whether in vitro embryo culture and transfer, which are manipulations of the nutritional environment during the periconceptional period, dysregulate postnatal blood pressure and blood pressure regulatory mechanisms. Embryos were either transferred to an intermediate recipient ewe (ET) or cultured in vitro in the absence (IVC) or presence of human serum (IVCHS) and a methyl donor (IVCHSþM) for 6 days. Basal blood pressure was recorded at 19–20 weeks after birth. Mean arterial pressure (MAP) and heart rate (HR) were measured before and after varying doses of phenylephrine (PE). mRNA expression of signaling molecules involved in blood pressure regulation was measured in the renal artery. Basal MAP did not differ between groups. Baroreflex sensitivity, set point, and upper plateau were also maintained in all groups after PE stimulation. Adrenergic receptors alpha-1A (αAR1A), alpha-1B (αAR1B), and angiotensin II receptor type 1 (AT1R) mRNA expression were not different from controls in the renal artery. These results suggest there is no programmed effect of ET or IVC on basal blood pressure or the baroreflex control mechanisms in adolescence, but future studies are required to determine the impact of ET and IVC on these mechanisms later in the life course when developmental programming effects may be unmasked by age. |
Keywords: | In vitro embryo culture; embryo transfer; artificial reproductive technologies; baroreflex; hypertension; cardiac; DOHaD |
Description: | First published online: 13 November 2020 |
Rights: | © The Author(s), 2020. Published by Cambridge University Press in association with International Society for Developmental Origins of Health and Disease. |
DOI: | 10.1017/S2040174420001014 |
Grant ID: | http://purl.org/au-research/grants/nhmrc/1066916 |
Published version: | http://dx.doi.org/10.1017/s2040174420001014 |
Appears in Collections: | Animal and Veterinary Sciences publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.