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https://hdl.handle.net/2440/13277
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Type: | Journal article |
Title: | Gene structure, alternative splicing, and chromosomal localization of pro-apoptotic Bcl-2 relative Bim |
Author: | Bouillet, P. Zhang, L. Huang, D. Webb, G. Bottema, C. Shore, P. Eyre, H. Sutherland, G. Adams, J. |
Citation: | Mammalian Genome, 2001; 12(2):163-168 |
Publisher: | Springer-Verlag |
Issue Date: | 2001 |
ISSN: | 0938-8990 1432-1777 |
Statement of Responsibility: | Philippe Bouillet, Li Chen Zhang, David C.S. Huang, Graham C. Webb, Cynthia D.K. Bottema, Paul Shore, Helen J. Eyre, Grant R. Sutherland, Jerry M. Adams |
Abstract: | Bim is a proapoptotic protein of the Bcl-2 family that shares only the short BH3 domain with other members. It has three isoforms, apparently produced by alternative splicing. The demonstration that Bim is essential for certain apoptotic responses and to prevent overproduction of hematopoietic cells suggests that it may be a tumor suppressor. We have, therefore, investigated the organization of the mouse Bim gene, delineating its promoter and splicing, and positioned the gene on both mouse and human chromosomes. Bim has six exons, but the third is a facultative intron that is spliced out in the mRNAs for the smaller isoforms (BimL and BimS), but not that encoding the largest isoform (BimEL). The 0.8-kb region 5' to exon 1, which contains a TATA-less promoter and binding sites for several transcription factors, can drive expression of a reporter gene. Mouse Bim localizes to the distal third of Chromosome (Chr) 2, near the F-G boundary, and its human counterpart to Chr 2q12 or q13. Deletions of these bands have been reported in ten tumors (eight hematopoietic), reinforcing the possibility that Bim is a tumor suppressor. These findings should help to clarify the regulation of Bim expression and to assess whether mutations involving Bim contribute to neoplastic and other diseases. |
Keywords: | Chromosomes, Human, Pair 2 Animals Humans Mice Carrier Proteins Membrane Proteins Proto-Oncogene Proteins Proto-Oncogene Proteins c-bcl-2 Protein Isoforms Blotting, Northern In Situ Hybridization, Fluorescence Physical Chromosome Mapping Reverse Transcriptase Polymerase Chain Reaction Apoptosis Alternative Splicing Base Sequence Introns Exons Molecular Sequence Data Male Apoptosis Regulatory Proteins Promoter Regions, Genetic Bcl-2-Like Protein 11 |
Description: | The original publication can be found at www.springerlink.com |
DOI: | 10.1007/s003350010242 |
Published version: | http://dx.doi.org/10.1007/s003350010242 |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest 7 |
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