Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/133221
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Type: | Journal article |
Title: | The BMP antagonist Gremlin1 contributes to the development of cortical excitatory neurons, motor balance and fear responses |
Author: | Suzuki, M. Suzuki, N. Wang, T. Kobayashi, H. Vrbanac, L. Ng, J.Q. Wright, J.A. Lannagan, T.R.M. Gieniec, K.A. Lewis, M. Ando, R. Enomoto, A. Koblar, S. Thomas, P. Worthley, D.L. Woods, S.L. |
Citation: | Development (Cambridge), 2021; 148(14):dev195883-1-dev195883-15 |
Publisher: | The Company of Biologists |
Issue Date: | 2021 |
ISSN: | 0950-1991 1477-9129 |
Statement of Responsibility: | Mari Ichinose, Nobumi Suzuki, Tongtong Wang, Hiroki Kobayashi, Laura Vrbanac, Jia Q. Ng, Josephine A. Wright, Tamsin R. M. Lannagan, Krystyna A. Gieniec, Martin Lewis, Ryota Ando, Atsushi Enomoto, Simon Koblar, Paul Thomas, Daniel L. Worthley and Susan L. Woods |
Abstract: | Bone morphogenetic protein (BMP) signaling is required for early forebrain development and cortical formation. How the endogenous modulators of BMP signaling regulate the structural and functional maturation of the developing brain remains unclear. Here we show that expression of the BMP antagonist, Grem1, marks committed layer Ⅴ and Ⅵ glutamatergic neurons in the embryonic mouse brain. Lineage tracing of Grem1-expressing cells in the embryonic brain was examined by administration of tamoxifen to pregnant Grem1creERT; Rosa26LSLTdtomato mice at 13.5 days post coitum (dpc), followed by collection of embryos later in gestation. In addition, at 14.5 dpc, bulk mRNA seq analysis of differentially expressed transcripts between FACS sorted Grem1 positive and negative cells was performed. We also generated Emx1-cre mediated Grem1 conditional knockout mice (Emx1-Cre;Grem1flox/flox) in which the Grem1 gene was deleted specifically in the dorsal telencephalon. Grem1Emx1cKO animals had reduced cortical thickness, especially layers Ⅴ and Ⅵ and impaired motor balance and fear sensitivity compared to littermate controls. This study has revealed new roles for Grem1 in the structural and functional maturation of the developing cortex. |
Keywords: | Grem1 BMP Cortical development Excitatory neuron Mouse |
Rights: | © 2021. Published by The Company of Biologists Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
DOI: | 10.1242/dev.195883 |
Grant ID: | http://purl.org/au-research/grants/nhmrc/CDF1 http://purl.org/au-research/grants/nhmrc/1140236 http://purl.org/au-research/grants/nhmrc/1099283 http://purl.org/au-research/grants/nhmrc/1143414 |
Published version: | http://dx.doi.org/10.1242/dev.195883 |
Appears in Collections: | Medicine publications |
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hdl_133221.pdf | Published version | 17.66 MB | Adobe PDF | View/Open |
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