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Type: Journal article
Title: Starch granule initiation and growth are altered in barley mutants that lack isoamylase activity
Author: Burton, R.
Jenner, H.
Carrangis, L.
Fahy, B.
Fincher, G.
Hylton, C.
Laurie, D.
Parker, M.
Waite, D.
Van Wegen, S.
Verhoeven, T.
Denyer, K.
Citation: Plant Journal, 2002; 31(1):97-112
Publisher: Blackwell Science Ltd
Issue Date: 2002
ISSN: 0960-7412
Statement of
Rachel A. Burton, Helen Jenner, Luke Carrangis, Brendan Fahy, Geoffrey B. Fincher, Chris Hylton, David A. Laurie, Mary Parker, Darren Waite, Sonja van Wegen, Tamara Verhoeven, Kay Denyer
Abstract: Two mutant lines of barley, Risø 17 and Notch-2, were found to accumulate phytoglycogen in the grain. Like the sugary mutants of maize and rice, these phytoglycogen-accumulating mutants of barley lack isoamylase activity in the developing endosperm. The mutants were shown to be allelic, and to have lesions in the isoamylase gene, isa1 that account for the absence of this enzyme. As well as causing a reduction in endosperm starch content, the mutations have a profound effect on the structure, number and timing of initiation of starch granules. There are no normal A-type or B-type granules in the mutants. The mutants have a greater number of starch granules per plastid than the wild-type and, particularly in Risø 17, this leads to the appearance of compound starch granules. These results suggest that, as well as suppressing phytoglycogen synthesis, isoamylase in the wild-type endosperm plays a role in determining the number, and hence the form, of starch granules.
Keywords: starch; barley; granules; initiation; isoamylase; phytoglycogen
Description: The definitive version is available at Article first published online: 1 JUL 2002
Rights: © 2002 Blackwell Science Ltd
RMID: 0020020849
DOI: 10.1046/j.1365-313X.2002.01339
Appears in Collections:Agriculture, Food and Wine publications

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