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Type: Journal article
Title: A single limit dextrinase gene is expressed both in the developing endosperm and in germinated grains of barley
Author: Burton, R.
Zhang, X.Q.
Hrmova, M.
Fincher, G.
Citation: Plant Physiology, 1999; 119(3):859-871
Issue Date: 1999
ISSN: 0032-0889
Statement of
Rachel A. Burton, Xiao-Qi Zhang, Maria Hrmova and Geoffrey B. Fincher
Abstract: The single gene encoding limit dextrinase (pullulan 6-glucanohy-drolase; EC in barley (Hordeum vulgare) has 26 introns that range in size from 93 to 822 base pairs. The mature polypeptide encoded by the gene has 884 amino acid residues and a calculated molecular mass of 97,417 D. Limit dextrinase mRNA is abundant in gibberellic acid-treated aleurone layers and in germinated grain. Gibberellic acid response elements were found in the promoter region of the gene. These observations suggest that the enzyme participates in starch hydrolysis during endosperm mobilization in germinated grain. The mRNA encoding the enzyme is present at lower levels in the developing endosperm of immature grain, a location consistent with a role for limit dextrinase in starch synthe-sis. Enzyme activity was also detected in developing grain. The limit dextrinase has a presequence typical of transit peptides that target nascent polypeptides to amyloplasts, but this would not be expected to direct secretion of the mature enzyme from aleurone cells in germinated grain. It remains to be discovered how the enzyme is released from the aleurone and whether another enzyme, possibly of the isoamylase group, might be equally important for starch hydrolysis in germinated grain.
Keywords: Hordeum
Glycoside Hydrolases
DNA, Plant
DNA, Complementary
DNA Primers
Reverse Transcriptase Polymerase Chain Reaction
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Plant
Amino Acid Sequence
Base Sequence
Sequence Homology, Amino Acid
Genes, Plant
Molecular Sequence Data
Promoter Regions, Genetic
DOI: 10.1104/pp.119.3.859
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Appears in Collections:Agriculture, Food and Wine publications
Aurora harvest 2

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