Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/138685
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Type: | Journal article |
Title: | Correlation between monkeypox viral load and infectious virus in clinical specimens |
Author: | Lim, C.K. McKenzie, C. Deerain, J. Chow, E.P.F. Towns, J. Chen, M.Y. Fairley, C.K. Tran, T. Williamson, D.A. |
Citation: | Journal of Clinical Virology, 2023; 161:105421-105421 |
Publisher: | Elsevier BV |
Issue Date: | 2023 |
ISSN: | 1386-6532 1873-5967 |
Statement of Responsibility: | Chuan Kok Lim, Charlene McKenzie, Joshua Deerain, Eric P.F. Chow, Janet Towns, Marcus Y Chen, Christopher K Fairley, Thomas Tran, Deborah A Williamson |
Abstract: | Background: In the 2022 mpox outbreak, several studies have explored longitudinal DNA shedding of mpox virus (MPXV) using PCR. However, there are fewer studies assessing infectivity in cell culture, and, by inference, MPXV transmissibility. Such information could help inform infection control and public health guidelines. Aims and Methods: The aim of this study was to correlate cell culture infectivity of clinical samples with viral loads in clinical samples. Between May to October 2022, clinical samples from different body sites sent to the Victorian Infectious Diseases Reference Laboratory in Melbourne, Australia for MPXV PCR detection were cultured in Vero cells as a surrogate for infectivity. Results: In the study period, 144 samples from 70 patients were tested by MPXV PCR. Viral loads in skin lesions were significantly higher than those in throat or nasopharyngeal samples (median Ct 22.0 vs 29.0, p = 0.0013 and median Ct 22.0 vs 36.5, p = 0.0001, respectively). Similarly, viral loads were significantly higher in anal samples compared to throat or nasopharyngeal samples (median Ct 20.0 vs. 29.0, p=<0.0001 and median Ct 20.0 vs. 36.5, p=<0.0001, respectively). Viral culture was successfully performed in 80/94 samples. Using logistic regression analysis, 50% of the samples were positive in viral culture at Ct 34.1 (95% confidence intervals 32.1–37.4). Conclusions: Our data further validate recent findings showing that samples with a higher MPXV viral load are more likely to demonstrate infectivity in cell culture. Although the presence of infectious virus in cell culture may not directly translate with clinical transmission risk, our data may be used as an adjunct help inform guidelines on testing and isolation policies in individuals with mpox. |
Keywords: | Mpox; Monkeypox virus; Viral culture; Viral load; Clinical specimens; Ct value |
Rights: | © 2023 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/bync- nd/4.0/). |
DOI: | 10.1016/j.jcv.2023.105421 |
Grant ID: | http://purl.org/au-research/grants/nhmrc/GNT1172873 http://purl.org/au-research/grants/nhmrc/GNT1172900 http://purl.org/au-research/grants/nhmrc/2022085 http://purl.org/au-research/grants/nhmrc/GNT1174555 http://purl.org/au-research/grants/nhmrc/HREC/79,322/MH-2021 |
Published version: | http://dx.doi.org/10.1016/j.jcv.2023.105421 |
Appears in Collections: | Medicine publications |
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hdl_138685.pdf | Published version | 471.71 kB | Adobe PDF | View/Open |
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