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https://hdl.handle.net/2440/14740
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Type: | Journal article |
Title: | Effects of Ca2+ concentration on GABAB receptor function in rat neocortical slices |
Author: | Ong, J. Kerr, D. |
Citation: | Canadian Journal of Physiology and Pharmacology, 2000; 78(6):507-511 |
Publisher: | Natl Research Council Canada |
Issue Date: | 2000 |
ISSN: | 0008-4212 1205-7541 |
Abstract: | In rat neocortical slices maintained in Mg2+-free Kreb's medium, the effects of Ca2+ concentration on repetitive spontaneous discharges and on GABA(B) receptor-mediated responses were investigated. Over a concentration range of 0.3-2.4 mM Ca2+, there was a reduction of discharge amplitude, with a 50 +/- 6.5% reduction in 0.3 mM Ca2+, whilst the burst frequency remained unaffected. Baclofen, the GABA(B) receptor agonist, produced a concentration-dependent depression of discharge frequency, reversibly antagonised by the antagonist (+)-(S)-5,5-dimethylmorpholinyl-2-acetic acid (Sch 50911). The EC50 value for baclofen in 2.4 mM Ca2+ was 11 microM, while the EC50 values in 0.3, 0.6, 1.2, and 1.8 mM Ca2+ were 1.3, 2.5, 3.6, and 10 microM, respectively, resulting in 8.5, 4.4, 3.1, and 1.1-fold leftward shifts. This enhanced action of baclofen in low extracellular Ca2+ concentrations in the neocortex may be the result of a lower concentration gradient which reinforces the action of baclofen. |
Keywords: | Neocortex Animals Rats Rats, Sprague-Dawley Calcium Chloride Baclofen Receptors, GABA-B GABA Agonists Synaptic Transmission Male |
DOI: | 10.1139/y00-017 |
Appears in Collections: | Anaesthesia and Intensive Care publications Aurora harvest 2 |
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