Please use this identifier to cite or link to this item:
Scopus Web of ScienceĀ® Altmetric
Type: Journal article
Title: Multiplexed microsatellite markers for the genetic analysis of Eucalyptus leucoxylon (Myrtaceae) and their utility for ecological and breeding studies in other Eucalyptus species
Author: Ottewell, K.
Donnellan, S.
Moran, G.
Paton, D.
Citation: Journal of Heredity, 2005; 96(4):445-451
Publisher: Oxford Univ Press Inc
Issue Date: 2005
ISSN: 0022-1503
Statement of
K. M. Ottewell, S. C. Donnellan, G. F. Moran, and D. C. Paton
Abstract: Eucalyptus leucoxylon is a widespread woodland tree species found in southeastern Australia that has suffered from, and continues to be, threatened by the impacts of habitat clearance and degradation. Populations now consist predominantly of scattered individuals, and their conservation status is of increasing concern. We report the development and characterization of a set of eight highly polymorphic microsatellite loci for E. leucoxylon. The loci can be amplified in three PCR multiplexes and electrophoresed in a single lane, allowing rapid throughput of large numbers of samples. A total of 111 alleles were detected in 68 individuals with an average of 12.3 alleles per locus, a mean expected heterozygosity of 0.83, and a mean observed heterozygosity of 0.72. The combined probabilities of identity and probabilities of paternity exclusion allow an extremely precise level of individual identification, indicating that these microsatellite markers will be ideal for population genetic and parentage-type studies in E. leucoxylon. The markers also exhibited an average of 76% conservation within the subgenus Symphyomyrtus, to which E. leucoxylon belongs, and 53% conservation across other subgenera of Eucalyptus, demonstrating the potential of these markers in ecological and breeding studies in a wide range of Eucalyptus species.
Keywords: Eucalyptus; DNA, Plant; DNA Primers; Genetic Markers; Crosses, Genetic; Ecosystem; Gene Amplification; Base Sequence; Microsatellite Repeats; Quantitative Trait Loci; Australia
RMID: 0020050778
DOI: 10.1093/jhered/esi057
Published version:
Appears in Collections:Earth and Environmental Sciences publications
Environment Institute publications

Files in This Item:
There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.