Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/16940
Citations | ||
Scopus | Web of ScienceĀ® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Identification of genes affecting glucose catabolism in nitrogen-limited fermentation |
Author: | Gardner, J. McBryde, C. Vystavelova, A. de Barros Lopes, M. Jiranek, V. |
Citation: | FEMS Yeast Research, 2005; 5(9):791-800 |
Publisher: | Elsevier Science BV |
Issue Date: | 2005 |
ISSN: | 1567-1356 1567-1364 |
Statement of Responsibility: | Jennifer M. Gardner, Colin McBryde, Andrea Vystavelova, Miguel De Barros Lopes and Vladimir Jiranek |
Abstract: | In recognition of the importance of assimilable nitrogen in the successful completion of several fermentation processes, we have sought to develop yeast strains that utilise this typically limited nutrient group more efficiently. With the aid of transposon mutagenesis together with a high-throughput method for analysis of multiple fermentations, we have identified nitrogen-efficient mutants that catabolise more sugar for a given amount of nitrogen utilised. In this way we have identified two genes, NGR1 and GID7, whose disruption leads to an enhanced catabolism of sugar in an industrial strain and/or a laboratory strain, during growth in a chemically defined grape juice medium with limiting nitrogen. Deletion of NGR1 or GID7 also resulted in minor changes in metabolites produced, and biomass yield, measured as dry weight, was also decreased in NGR1 mutant strains. |
Keywords: | Assimilable nitrogen Wine Transposon mutagenesis Yeast Fermentation Industrial biotechnology |
Description: | The definitive version is available at www.blackwell-synergy.com |
DOI: | 10.1016/j.femsyr.2005.02.008 |
Published version: | http://www.blackwell-synergy.com/doi/abs/10.1016/j.femsyr.2005.02.008 |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest 2 |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.