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Type: Journal article
Title: Identification of genes affecting glucose catabolism in nitrogen-limited fermentation
Author: Gardner, J.
McBryde, C.
Vystavelova, A.
de Barros Lopes, M.
Jiranek, V.
Citation: FEMS Yeast Research, 2005; 5(9):791-800
Publisher: Elsevier Science BV
Issue Date: 2005
ISSN: 1567-1356
Statement of
Jennifer M. Gardner, Colin McBryde, Andrea Vystavelova, Miguel De Barros Lopes and Vladimir Jiranek
Abstract: In recognition of the importance of assimilable nitrogen in the successful completion of several fermentation processes, we have sought to develop yeast strains that utilise this typically limited nutrient group more efficiently. With the aid of transposon mutagenesis together with a high-throughput method for analysis of multiple fermentations, we have identified nitrogen-efficient mutants that catabolise more sugar for a given amount of nitrogen utilised. In this way we have identified two genes, NGR1 and GID7, whose disruption leads to an enhanced catabolism of sugar in an industrial strain and/or a laboratory strain, during growth in a chemically defined grape juice medium with limiting nitrogen. Deletion of NGR1 or GID7 also resulted in minor changes in metabolites produced, and biomass yield, measured as dry weight, was also decreased in NGR1 mutant strains.
Keywords: Assimilable nitrogen; Wine; Transposon mutagenesis; Yeast; Fermentation; Industrial biotechnology
Description: The definitive version is available at
RMID: 0020050521
DOI: 10.1016/j.femsyr.2005.02.008
Published version:
Appears in Collections:Agriculture, Food and Wine publications

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