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Type: Journal article
Title: Molecular identification and detection of Eutypa lata in grapevine
Author: Lardner, R.
Stummer, B.
Sosnowski, M.
Scott, E.
Citation: Mycological Research, 2005; 109(7):799-808
Publisher: Cambridge Univ Press
Issue Date: 2005
ISSN: 0953-7562
Abstract: Eutypa lata, the causal agent of Eutypa dieback of grapevines, is difficult to identify on the basis of colony morphology and is often out-competed by other fungi when isolated from wood. To facilitate diagnosis of the pathogen, we designed SCAR primers capable of amplifying DNA of E. lata and constructed a genomic DNA library from which DNA sequences specific to E. lata were identified and sequenced. SCAR primers were used to identify E. lata directly from culture without the requirement for DNA extraction or prolonged incubation periods and could also detect the pathogen in DNA isolated from grapevine wood. RFLP probes were used in slot-blot assays to detect the pathogen in DNA isolated from 1 yr old cane as well as from mature grapevine trunks. The markers developed in this study have the potential to be used as a research tool to gather information on the epidemiology of the disease and to assess the efficacy of potential control agents against E. lata.
Keywords: Ascomycota; Vitis; DNA, Fungal; Plant Diseases; Polymorphism, Restriction Fragment Length
RMID: 0020050954
DOI: 10.1017/S0953756205002893
Appears in Collections:Agriculture, Food and Wine publications

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