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Type: Journal article
Title: Platelet nitrate responsiveness in fasting and postprandial type 2 diabetes
Author: Anderson, R.
Ellis, G.
Evans, L.
Morris, K.
Chirkov, Y.
Horowitz, J.
Jackson, S.
Rees, A.
Lewis, M.
Frenneaux, M.
Citation: Diabetes and Vascular Disease Research, 2005; 2(2):88-93
Publisher: MediNews (Diabetes) Ltd.
Issue Date: 2005
ISSN: 1479-1641
Statement of
Richard A Anderson, Gethin R Ellis, L Marc Evans, Keith Morris, Yuri Y Chirkov, John D Horowitz, Simon K Jackson, Alan Rees, Malcolm J Lewis, Michael P Frenneaux
Abstract: Vascular responsiveness to exogenous nitrates in type 2 diabetes (T2DM) is attenuated in brachial and coronary vessels. We determined platelet responsiveness to nitric oxide (NO) in T2DM and control subjects. We examined whether the postprandial (PP) state affected platelet sensitivity to NO donors in T2DM patients and the extent of correlation between this and measures of oxidative stress, compared to changes in endothelial function. Twelve T2DM subjects were studied fasting and four hours after a test meal and compared with 15 healthy controls. We assessed the inhibitory effects of NO donors on adenosine 5'-diphosphate (ADP)-induced platelet aggregation. Oxidative stress was assessed by lipid-derived free radicals, ex vivo by electron paramagnetic resonance spectroscopy and markers of lipid peroxidation. Endothelial function was assessed by flow-mediated vasodilatation (FMD) of the brachial artery. Results are expressed as (mean +/- SEM). Fasting platelet aggregation was increased in diabetics versus controls (14.86 +/- 1.1 Ohms vs. 10.76 +/- 1.1 Ohms, p < 0.05). Sodium nitroprusside (SNP) and glyceryl trinitrate (GTN) inhibited ADP-induced aggregation by 73.1 +/- 5.9% and 50.3 +/- 7.7% in healthy controls compared to 15.4 +/- 7% and 19.5 +/- 8.2% in T2DM (p < 0.05). Fasting and postprandial inhibition of platelet aggregation with NO donors in T2DM was similar. T2DM patients had higher levels of oxidative stress in the fasting state and postprandially. There were no PP correlations with platelet NO resistance. In conclusion, there is platelet hyporesponsiveness to NO donors (SNP/GTN) in T2DM compared to controls, with increased ADP-induced platelet aggregation. Platelet abnormalities were associated with increased oxidative stress.
Keywords: Brachial Artery
Blood Platelets
Diabetes Mellitus, Type 2
Free Radicals
Adenosine Diphosphate
Nitric Oxide Donors
Lipid Peroxidation
Oxidative Stress
Platelet Aggregation
Postprandial Period
Time Factors
Middle Aged
Description: © Sherborne Gibbs
DOI: 10.3132/dvdr.2005.015
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