Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/23190
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dc.contributor.authorJesudason, S.en
dc.contributor.authorHumphreys, I.en
dc.contributor.authorKireta, S.en
dc.contributor.authorGilchrist, R.en
dc.contributor.authorBardy, P.en
dc.contributor.authorRuss, G.en
dc.contributor.authorCoates, P.en
dc.date.issued2006en
dc.identifier.citationJournal of Immunological Methods, 2006; 314(1-2):153-163en
dc.identifier.issn0022-1759en
dc.identifier.issn1872-7905en
dc.identifier.urihttp://hdl.handle.net/2440/23190-
dc.descriptionCopyright © 2006 Elsevier B.V. All rights reserved.en
dc.description.abstractThe common marmoset (Callithrix jacchus) is emerging as a promising alternative pre-clinical model for transplantation and immunological research. It is therefore important to establish a rapid and reliable method of confirming alloreactivity between donor–recipient pairs. In this study of a large marmoset colony (n = 49), we firstly characterised MHC Class II genes (Caja-DRBW1201, Caja-DRB103, Caja-DRBW16) using, for the first time in this species, sequence-based allelic typing techniques. Exon 2 was amplified using M13-tailed PCR primers specific for known marmoset alleles, and sequenced using universal M13 sequencing primers and dye terminator cycle sequencing. Twenty-six genotypes involving monomorphic Caja-DRBW1201, 8 Caja-DRBW16 and 5 Caja-DRB103 alleles were observed. Two new DRBW16 alleles were identified. Subsequently we investigated whether matching at MHC-DRB loci alone could accurately predict in-vitro alloreactivity as assessed by mixed lymphocyte reactions. Peripheral blood mononuclear cells (PBMC) isolated from fully and partially DRB-matched and fully mismatched animal pairs were mixed and co-cultured for T-cell proliferation. PBMC co-cultured from fully or partially mismatched pairs exhibited significant T cell proliferation above single cell controls (p < 0.01). Mixed PBMC from fully DRB-matched pairs exhibited no proliferation over controls (p = 0.3). Thus using Caja-DRB genotyping, suitably alloreactive donor–recipient pairs can be rapidly and accurately identified for use in further studies of cellular and solid organ transplantation.en
dc.description.statementofresponsibilityShilpanjali Prasad, Ian Humphreys, Svjetlana Kireta, Robert Bruce Gilchrist, Peter Bardy, Graeme R. Russ and Patrick Toby Hewlett Coatesen
dc.description.urihttp://www.sciencedirect.com/science/journal/00221759en
dc.language.isoenen
dc.publisherElsevier Science BVen
dc.subjectcommon marmoset; major histocompatibility complex; mixed lymphocyte reaction; chimerism; transplantation; non-human primateen
dc.titleMHC Class II DRB genotyping is highly predictive of in-vitro alloreactivity in the common marmoseten
dc.typeJournal articleen
dc.identifier.rmid0020061319en
dc.identifier.doi10.1016/j.jim.2006.06.009en
dc.identifier.pubid52362-
pubs.library.collectionObstetrics and Gynaecology publicationsen
pubs.verification-statusVerifieden
pubs.publication-statusPublisheden
dc.identifier.orcidJesudason, S. [0000-0001-9695-0761]en
dc.identifier.orcidGilchrist, R. [0000-0003-1611-7142]en
Appears in Collections:Obstetrics and Gynaecology publications

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