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https://hdl.handle.net/2440/23198
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Type: | Journal article |
Title: | Essential role for IL-10 in resistance to lipopolysaccharide-induced preterm labor in mice |
Author: | Robertson, S. Skinner, R. Care, A. |
Citation: | Journal of Immunology, 2006; 177(7):4888-4896 |
Publisher: | Amer Assoc Immunologists |
Issue Date: | 2006 |
ISSN: | 0022-1767 1550-6606 |
Statement of Responsibility: | Sarah A. Robertson, Rebecca J. Skinner, and Alison S. Care |
Abstract: | IL-10 is highly expressed in the uterus and placenta and is implicated in controlling inflammation-induced pathologies of pregnancy. To investigate the role of IL-10 in regulating preterm labor, the response of IL-10 null mutant mice to low-dose LPS in late gestation was evaluated. When IL-10 null mutant C57BL/6 (IL-10–/–) and control (IL-10+/+) mice were administered LPS on day 17 of pregnancy, the dose of LPS required to elicit 50% preterm fetal loss was 10-fold lower in IL-10–/– mice than in IL-10+/+ mice. Surviving fetuses in IL-10–/– mice exhibited fetal growth restriction at lower doses of LPS than IL-10+/+ mice. Marked elevation of LPS-induced immunoactive TNF- and IL-6 was evident in the serum, uterus, and placenta of IL-10–/– mice, and TNF- and IL-6 mRNA expression was elevated in the uterus and placenta, but not the fetus. Serum IL-1, IFN-, and IL-12p40 were increased and soluble TNFRII was diminished in the absence of IL-10, with these changes also reflected in the gestational tissues. Administration of rIL-10 to IL-10–/– mice attenuated proinflammatory cytokine synthesis and alleviated their increased susceptibility to preterm loss. Exogenous IL-10 also protected IL-10+/+ mice from fetal loss. These data show that IL-10 modulates resistance to inflammatory stimuli by down-regulating proinflammatory cytokines in the uterus and placenta. Abundance of endogenous IL-10 in gestational tissues is therefore identified as a critical determinant of resistance to preterm labor, and IL-10 may provide a useful therapeutic agent in this common condition. |
Keywords: | Uterus Placenta Animals Mice Mice, Mutant Strains Inflammation Lipopolysaccharides Tumor Necrosis Factor-alpha Receptors, Tumor Necrosis Factor, Type II Protein Subunits RNA, Messenger Interleukin-1 Interleukin-6 Interleukin-10 Interleukin-12 Immunoassay Reverse Transcriptase Polymerase Chain Reaction Pregnancy Mutation Female Obstetric Labor, Premature Interleukin-12 Subunit p40 Interferon-gamma |
Description: | Copyright © 2006 by The American Association of Immunologists, Inc. |
DOI: | 10.4049/jimmunol.177.7.4888 |
Published version: | http://www.jimmunol.org/cgi/content/abstract/177/7/4888 |
Appears in Collections: | Aurora harvest 6 Obstetrics and Gynaecology publications |
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