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Type: Journal article
Title: Differential regulation of suppressor of cytokine signaling-3 in the liver and adipose tissue of the sheep fetus in late gestation
Author: Gentili, S.
Waters, M.
McMillen, I.
Citation: American Journal of Physiology-Regulatory Integrative and Comparative Physiology, 2006; 290(4):R1044-R1051
Publisher: Amer Physiological Soc
Issue Date: 2006
ISSN: 0363-6119
Abstract: It is unknown whether the JAK/STAT/suppressor of cytokine signaling-3 (SOCS-3) intracellular signaling pathway plays a role in tissue growth and metabolism during fetal life. We investigated whether there is a differential profile of SOCS-3 expression in the liver and perirenal adipose tissue during the period of increased fetal growth in late gestation and the impact of fetal growth restriction on SOCS-3 expression in the fetal liver. We also determined whether basal SOCS-3 expression in the fetal liver and perirenal adipose tissue is regulated by endogenous fetal prolactin (PRL). SOCS-3 mRNA abundance was higher in the liver than in the pancreas, spleen, and kidney of the sheep fetus during late gestation. In the liver, SOCS-3 mRNA expression was increased (P < 0.05) between 125 (n = 4) and 145 days (n = 7) gestation and lower (P < 0.05) in growth-restricted compared with normally grown fetal sheep in late gestation. The relative expression of SOCS-3 mRNA in the fetal liver was directly related to the mean plasma PRL concentrations during a 48-h infusion of either a dopaminergic agonist, bromocriptine (n = 7), or saline (n = 5), such that SOCS-3 mRNA expression was lower when plasma PRL concentrations decreased below approximately 20 ng/ml [y = 0.99 - (2.47/x) + (4.96/x(2)); r(2) = 0.91, P < 0.0001, n = 12]. No relationship was shown between the abundance of phospho-STAT5 in the fetal liver and circulating PRL. SOCS-3 expression in perirenal adipose tissue decreased (P < 0001) between 90-91 (n = 6) and 140-145 days (n = 9) gestation and was not related to endogenous PRL concentrations. Thus SOCS-3 is differentially expressed and regulated in key fetal tissues and may play an important and tissue-specific role in the regulation of cellular proliferation and differentiation before birth.
Keywords: Liver; Adipose Tissue; Placenta; Animals; Sheep; Bromocriptine; Prolactin; RNA, Messenger; Signal Transduction; Gene Expression Regulation, Developmental; Gestational Age; STAT5 Transcription Factor; Suppressor of Cytokine Signaling Proteins; Suppressor of Cytokine Signaling 3 Protein
RMID: 0020060093
DOI: 10.1152/ajpregu.00573.2005
Appears in Collections:Molecular and Biomedical Science publications

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