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https://hdl.handle.net/2440/27609
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Type: | Journal article |
Title: | Tetraploid embryonic stem cells contribute to the inner cell mass of mouse blastocysts |
Author: | Pralong, D. Lim, M. Vassiliev, I. Mrozik, K. Wijesundara, N. Rathjen, P. Verma, P. |
Citation: | Cellular Reprogramming, 2005; 7(4):272-278 |
Publisher: | Mary Ann Liebert, Inc. Publishers |
Issue Date: | 2005 |
ISSN: | 1536-2302 1557-7457 |
Organisation: | Centre for the Molecular Genetics of Development |
Statement of Responsibility: | Daniele Pralong, Mei Ling Lim, Ivan Vassiliev, Krzysztof Mrozik, Nishanthi Wijesundara, Peter Rathjen, Paul J. Verma |
Abstract: | The demonstration that mouse somatic cells can be reprogrammed following fusion with embryonic stem (ES) cells may provide an alternative to somatic cell nuclear transfer (therapeutic cloning) to generate autologous stem cells. In an attempt to produce cells with an increased pool of reprogramming factors, tetraploid ES cells were produced by polyethylene glycol mediated fusion of two ES cell lines transfected with plasmids carrying puromycin or neomycin resistance cassettes, respectively, followed by double antibiotic selection. Tetraploid ES cells retain properties characteristic of diploid ES cells, including the expression of pluripotent gene markers Oct4 and Rex1. On injection into the testis capsule of severe combined immunodeficient (SCID) mice, tetraploid ES cells are able to form teratomas containing cells representative of all three germ layers. Further, these cells demonstrated the ability to integrate into the inner cell mass of blastocysts. This study indicates that tetraploid ES cells are promising candidates as cytoplasm donors for reprogramming studies. |
Keywords: | Testis Cells, Cultured Stem Cells Blastocyst Germ Layers Animals Mice Mice, SCID Teratoma Stem Cell Transplantation Polyploidy Male Octamer Transcription Factor-3 |
DOI: | 10.1089/clo.2005.7.272 |
Description (link): | http://www.ncbi.nlm.nih.gov/pubmed/16390263 |
Published version: | http://dx.doi.org/10.1089/clo.2005.7.272 |
Appears in Collections: | Aurora harvest 2 Centre for the Molecular Genetics of Development publications Molecular and Biomedical Science publications |
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