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Type: Journal article
Title: Tetraploid embryonic stem cells contribute to the inner cell mass of mouse blastocysts
Author: Pralong, D.
Lim, M.
Vassiliev, I.
Mrozik, K.
Wijesundara, N.
Rathjen, P.
Verma, P.
Citation: Cloning and Stem Cells, 2005; 7(4):272-278
Publisher: Mary Ann Liebert, Inc. Publishers
Issue Date: 2005
ISSN: 1536-2302
Organisation: Centre for the Molecular Genetics of Development
Statement of
Daniele Pralong, Mei Ling Lim, Ivan Vassiliev, Krzysztof Mrozik, Nishanthi Wijesundara, Peter Rathjen, Paul J. Verma
Abstract: The demonstration that mouse somatic cells can be reprogrammed following fusion with embryonic stem (ES) cells may provide an alternative to somatic cell nuclear transfer (therapeutic cloning) to generate autologous stem cells. In an attempt to produce cells with an increased pool of reprogramming factors, tetraploid ES cells were produced by polyethylene glycol mediated fusion of two ES cell lines transfected with plasmids carrying puromycin or neomycin resistance cassettes, respectively, followed by double antibiotic selection. Tetraploid ES cells retain properties characteristic of diploid ES cells, including the expression of pluripotent gene markers Oct4 and Rex1. On injection into the testis capsule of severe combined immunodeficient (SCID) mice, tetraploid ES cells are able to form teratomas containing cells representative of all three germ layers. Further, these cells demonstrated the ability to integrate into the inner cell mass of blastocysts. This study indicates that tetraploid ES cells are promising candidates as cytoplasm donors for reprogramming studies.
Keywords: Testis; Cells, Cultured; Stem Cells; Blastocyst; Germ Layers; Animals; Mice; Mice, SCID; Teratoma; Stem Cell Transplantation; Polyploidy; Male; Octamer Transcription Factor-3
RMID: 0020052734
DOI: 10.1089/clo.2005.7.272
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Appears in Collections:Molecular and Biomedical Science publications
Centre for the Molecular Genetics of Development publications

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