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https://hdl.handle.net/2440/28044
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Type: | Journal article |
Title: | Structural and promoter regions of the murine pyruvate carboxylase gene |
Author: | Jitrapakdee, S. Petchamphai, N. Sunyakumthorn, P. Wallace, J. Boonsaeng, V. |
Citation: | Biochemical and Biophysical Research Communications, 2001; 287(2):411-417 |
Publisher: | Academic Press Inc |
Issue Date: | 2001 |
ISSN: | 0006-291X |
Statement of Responsibility: | Sarawut Jitrapakdee; Nathida Petchamphai; Piyanate Sunyakumthorn; John C. Wallace; Vichai Boonsaeng |
Abstract: | We have cloned and sequenced the gene encoding mouse pyruvate carboxylase (mPC) [EC 6.4.1.1]. The coding region contains 19 exons, one 5′-untranslated region exon, and 19 introns in 22 kb of genomic DNA. This gene's exon/intron organization is highly conserved with respect to rat and human PC genes. The mPC gene promoter lacks canonical TATA and CCAAT boxes, in common with a number of housekeeping genes. Transient expressions in COS-1 of a luciferase reporter gene under the control of 5′-nested deletions of the 5′-flanking sequence of the mPC gene have identified the 166-bp minimal sequence required for basal transcription. Alternative splicing at the 5′-untranslated region exon of the mouse PC gene results in the production of two alternate transcripts bearing different 5′-noncoding regions. Both transcripts are highly expressed in kidney and liver and moderately expressed in heart and testis and expressed at a low level in spleen. |
Keywords: | pyruvate carboxylase biotin-dependent enzyme gluconeogenesis lipogenesis gene regulation gene structure |
Rights: | © 2001 Academic Press. All rights reserved. |
DOI: | 10.1006/bbrc.2001.5599 |
Published version: | http://dx.doi.org/10.1006/bbrc.2001.5599 |
Appears in Collections: | Aurora harvest 6 Molecular and Biomedical Science publications |
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