Please use this identifier to cite or link to this item:
Scopus Web of Science® Altmetric
Type: Journal article
Title: Production of chemokines in vivo in response to microbial stimulation
Author: Coates, N.
McColl, S.
Citation: Journal of Immunology, 2001; 166(8):5176-5182
Publisher: Amer Assoc Immunologists
Issue Date: 2001
ISSN: 0022-1767
Statement of
Nicholas J. Coates and Shaun R. McColl
Abstract: Members of the chemokine gene superfamily are known to play a central role in leukocyte extravasation; however, their involvement in acute inflammation in response to micro-organisms has not yet been well studied. We have therefore investigated the role of murine macrophage-inflammatory protein (muMIP) 1 and muMIP-2 in the inflammatory response mounted against the bacteria Salmonella enteritidis and the Sacchromyces cerevisiae cell wall component, zymosan. Leukocyte extravasation was monitored in murine s.c. air pouches. Both agonists induced accumulation of leukocytes in a dose- and time-dependent manner, with the response peaking after 4 h and declining thereafter. The inflammatory exudate comprised mainly neutrophils; however, an increase in eosinophil accumulation was also observed in response to zymosan. The production of both muMIP-1 and muMIP-2 increased with time in response to both the agonists, although production was more sustained in response to the bacteria. Prior treatment of mice with neutralizing Abs against muMIP-1 or muMIP-2, either alone or in combination, failed to attenuate the accumulation of leukocytes in response to the agonists. In contrast, the anti-muMIP-2 Abs significantly inhibited leukocyte recruitment in response to S. enteritidis in complement-deficient mice. Taken together, these data show that while muMIP-1 and muMIP-2 are produced in response to phagocytosis of micro-organisms in s.c. tissue, under these circumstances components of the complement pathway appear to play a dominant role in the recruitment of neutrophils.
Keywords: Phagocytes
Exudates and Transudates
Mice, Inbred BALB C
Mice, Mutant Strains
Salmonella enteritidis
Saccharomyces cerevisiae
Sodium Chloride
Macrophage Inflammatory Proteins
Immune Sera
Diffusion Chambers, Culture
Injections, Intraperitoneal
Injections, Subcutaneous
Cell Movement
Complement C5
Chemokine CCL4
Chemokine CXCL2
Description: Copyright © 2001 by The American Association of Immunologists
DOI: 10.4049/jimmunol.166.8.5176
Published version:
Appears in Collections:Aurora harvest 2
Molecular and Biomedical Science publications

Files in This Item:
There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.