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|Title:||Dietary supplementation with zinc and a growth factor extract derived from bovine cheese whey improves methotrexate-damaged rat intestine|
|Citation:||American Journal of Clinical Nutrition, 2003; 77(5):1296-1303|
|Publisher:||Amer Soc Clinical Nutrition|
|Cuong D Tran, Gordon S Howarth, Peter Coyle, Jeffrey C Philcox, Allan M Rofe, and Ross N Butler|
|Abstract:||Background: Oral administration of zinc or bovine whey-derived growth factor extract (WGFE) is known to reduce intestinal permeability and ameliorate methotrexate (MTX)-induced mucositis, respectively. Objective: We examined the effects of zinc, WGFE, and zinc plus WGFE on gut damage in MTX-treated rats. Design: Rats (n = 16/group) were fed zinc (1000 mg/kg diet), WGFE (32 mg/kg diet), zinc plus WGFE, or control (10 mg Zn/kg diet) diets for 7 d and then injected subcutaneously with MTX (2.5 mg/kg) for 3 d to induce gut damage. Gut histology and intestinal permeability were assessed. Results: The Zn+WGFE diet was associated with both reduced gut damage on day 5 and enhanced recovery on day 7. The WGFE diet ameliorated gut damage, whereas the Zn and Zn+WGFE diets enhanced repair. Gut metallothionein and tissue zinc concentrations were significantly (P < 0.01) higher with Zn and Zn+WGFE on days 5 and 7 than without zinc supplementation. The Zn and Zn+WGFE diets significantly (P < 0.05) decreased gut permeability on days 3–4 compared with the control diet. Intestinal permeability was significantly (P < 0.05) increased on days 5–6. On days 6–7, only the WGFE diet improved gut permeability (by 80%) compared with the control diet. Conclusions: Dietary administration of WGFE and a pharmacologic dose of zinc reduced intestinal damage and enhanced recovery, respectively. WGFE also improved gut permeability after MTX-induced bowel damage. In combination, zinc and WGFE hastened repair of gut damage, which may have clinical application in chemotherapy-induced mucositis.|
|Keywords:||Zinc; whey growth factor extract; intestinal permeability; small-intestine damage|
|Appears in Collections:||Molecular and Biomedical Science publications|
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