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Type: Journal article
Title: Antimicrobial peptides from amphibian skin potently inhibit human immunodeficiency virus infection and transfer of virus from dendritic cells to T cells
Author: VanCompernolle, S.
Taylor, R.
Oswald-Richter, K.
Jiang, J.
Youree, B.
Bowie, J.
Tyler, M.
Conlon, J.
Wade, D.
Aiken, C.
Dermody, T.
KewalRamani, V.
Rollins-Smith, L.
Unutmaz, D.
Citation: Journal of Virology, 2005; 79(18):11598-11606
Publisher: Amer Soc Microbiology
Issue Date: 2005
ISSN: 0022-538X
Statement of
Scott E. VanCompernolle, R. Jeffery Taylor, Kyra Oswald-Richter, Jiyang Jiang, Bryan E. Youree, John H. Bowie, Michael J. Tyler, J. Michael Conlon, David Wade, Christopher Aiken, Terence S. Dermody, Vineet N. KewalRamani, Louise A. Rollins-Smith, and Derya Unutmaz
Abstract: Topical antimicrobicides hold great promise in reducing human immunodeficiency virus (HIV) transmission. Amphibian skin provides a rich source of broad-spectrum antimicrobial peptides including some that have antiviral activity. We tested 14 peptides derived from diverse amphibian species for the capacity to inhibit HIV infection. Three peptides (caerin 1.1, caerin 1.9, and maculatin 1.1) completely inhibited HIV infection of T cells within minutes of exposure to virus at concentrations that were not toxic to target cells. These peptides also suppressed infection by murine leukemia virus but not by reovirus, a structurally unrelated nonenveloped virus. Preincubation with peptides prevented viral fusion to target cells and disrupted the HIV envelope. Remarkably, these amphibian peptides also were highly effective in inhibiting the transfer of HIV by dendritic cells (DCs) to T cells, even when DCs were transiently exposed to peptides 8 h after virus capture. These data suggest that amphibian-derived peptides can access DC-sequestered HIV and destroy the virus before it can be transferred to T cells. Thus, amphibian-derived antimicrobial peptides show promise as topical inhibitors of mucosal HIV transmission and provide novel tools to understand the complex biology of HIV capture by DCs.
Keywords: Dendritic Cells
Cells, Cultured
HIV Infections
Antimicrobial Cationic Peptides
Amino Acid Sequence
Molecular Sequence Data
Immunity, Innate
In Vitro Techniques
Description: © 2005 American Society for Microbiology
DOI: 10.1128/JVI.79.18.11598-11606.2005
Published version:
Appears in Collections:Aurora harvest 2
Chemistry publications

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