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https://hdl.handle.net/2440/32459
Type: | Journal article |
Title: | Identification and partial characterization of glycosidic activities of commercial strains of the lactic acid bacterium, Oenococcus oeni |
Author: | Grimaldi, A. McLean, H. Jiranek, V. |
Citation: | American Journal of Enology and Viticulture, 2000; 51(4):362-369 |
Publisher: | American Society of Enologists and Viticulture |
Issue Date: | 2000 |
ISSN: | 0002-9254 1943-7749 |
Statement of Responsibility: | Antonio Grimaldi, Hylton McLean and Vladimir Jiranek |
Abstract: | β-glucosidase activity was measured in cultures derived from 11 commercial preparations of Oenococcus oeni, which are utilized for the malolactic fermentation of wine. All cultures produced a detectable activity seen as the hydrolysis of p-nitrophenyl-ß-D-glucopyranoside. Detectable activities against p-nitrophenyl-ß-D-xylopyranoside and, to a lesser extent for selected isolates, p-nitrophenyl-α-L-arabinopyranoside, were also observed. The activity occurred in both the supernatant and cell-associated fractions, with the combined total activity and its relative distribution varying according to strain and growth stage. In evaluating the potential of these activities as liberators of glyco-conjugated grape aromas, responses to enological pH values, glucose/ fructose and ethanol concentrations were determined. Over the pH range of 3.5 to 4.0, 12% to 43% of the activity observed at the optimal pH of 5.5 was retained. Depending on the isolate examined, the presence of up to 166 mM sugar yielded responses ranging between 30% enhancement and 93% inhibition. Similarly, responses to ethanol ranged from 80% enhancement to 73% inhibition. When examined in a combinatorial manner, a heightened inhibition by some pH/sugar/ethanol combinations indicated a synergism for some variables. The potential enological significance of these findings is discussed. |
Rights: | © 2000 by the American Society for Enology and Viticulture |
Published version: | http://www.ajevonline.org/content/51/4/362.full.pdf+html |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest |
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