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Type: Journal article
Title: SNARE-protein-mediated disease resistance at the plant cell wall
Author: Collins, N.
Thordal-Christensen, H.
Lipka, V.
Bau, S.
Kombrink, E.
Qiu, J.
Huckelhoven, R.
Stein, M.
Freialdenhoven, A.
Somerville, S.
Schulze-Lefert, P.
Citation: Nature, 2003; 425(6961):973-977
Publisher: Nature Publishing Group
Issue Date: 2003
ISSN: 0028-0836
Statement of
Nicholas C. Collins, Hans Thordal-Christensen, Volker Lipka, Stephan Bau, Erich Kombrink, Jin-Long Qiu, Ralph Hückelhoven, Mónica Stein, Andreas Freialdenhoven, Shauna C. Somerville and Paul Schulze-Lefert
Abstract: Failure of pathogenic fungi to breach the plant cell wall constitutes a major component of immunity of non-host plant species—species outside the pathogen host range—and accounts for a proportion of aborted infection attempts on 'susceptible' host plants (basal resistance).Neither form of penetration resistance is understood at the molecular level. We developed a screen for penetration (pen) mutants of Arabidopsis, which are disabled in non-host penetration resistance against barley powdery mildew, Blumeria graminis f. sp. hordei, and we isolated the PEN1 gene. We also isolated barley ROR2 , which is required for basal penetration resistance against B. g. hordei. The genes encode functionally homologous syntaxins, demonstrating a mechanistic link between non-host resistance and basal penetration resistance in monocotyledons and dicotyledons. We show that resistance in barley requires a SNAP-25 (synaptosome-associated protein, molecular mass 25 kDa) homologue capable of forming a binary SNAP receptor (SNARE) complex with ROR2. Genetic control of vesicle behaviour at penetration sites, and plasma membrane location of PEN1/ROR2, is consistent with a proposed involvement of SNARE-complex-mediated exocytosis and/or homotypic vesicle fusion events in resistance. Functions associated with SNARE-dependent penetration resistance are dispensable for immunity mediated by race-specific resistance (R) genes, highlighting fundamental differences between these two resistance forms.
Keywords: Cell Wall
Membrane Proteins
Vesicular Transport Proteins
Nerve Tissue Proteins
Arabidopsis Proteins
Cloning, Molecular
Two-Hybrid System Techniques
Plant Diseases
Protein Binding
Molecular Sequence Data
SNARE Proteins
Qa-SNARE Proteins
Synaptosomal-Associated Protein 25
DOI: 10.1038/nature02076
Appears in Collections:Aurora harvest
Australian Centre for Plant Functional Genomics publications

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