Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/34551
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Type: Journal article
Title: Ectopic Expression of atRSZ33 Reveals Its Function in Splicing and Causes Pleiotropic Changes in Development
Author: Kalyna, M.
Lopato, S.
Barta, A.
Citation: Molecular Biology of the Cell, 2003; 14(9):3565-3577
Publisher: Amer Soc Cell Biology
Issue Date: 2003
ISSN: 1059-1524
1939-4586
Statement of
Responsibility: 
Maria Kalyna, Sergiy Lopato, and Andrea Barta
Abstract: Splicing provides an additional level in the regulation of gene expression and contributes to proteome diversity. Herein, we report the functional characterization of a recently described plant-specific protein, atRSZ33, which has characteristic features of a serine/arginine-rich protein and the ability to interact with other splicing factors, implying that this protein might be involved in constitutive and/or alternative splicing. Overexpression of atRSZ33 leads to alteration of splicing patterns of atSRp30 and atSRp34/SR1, indicating that atRSZ33 is indeed a splicing factor. Moreover, atRSZ33 is a regulator of its own expression, as splicing of its pre-mRNA is changed in transgenic plants. Investigations by promoter--glucuronidase (GUS) fusion and in situ hybridization revealed that atRSZ33 is expressed during embryogenesis and early stages of seedling formation, as well as in flower and root development. Ectopic expression of atRSZ33 caused pleiotropic changes in plant development resulting in increased cell expansion and changed polarization of cell elongation and division. In addition, changes in activity of an auxin-responsive promoter suggest that auxin signaling is disturbed in these transgenic plants.
RMID: 0020063334
DOI: 10.1091/mbc.E03-02-0109
Appears in Collections:Agriculture, Food and Wine publications

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