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Type: Journal article
Title: Chicken microchromosomes are hypermethylated and can be identified by specific painting probes
Author: Grutzner, F.
Tsend-Ayush, E.
Stout, K.
Munsche, S.
Niveleau, A.
Nanda, I.
Schmid, M.
Haaf, T.
Citation: Cytogenetic and Genome Research, 2001; 93(3-4):265-269
Publisher: Karger
Issue Date: 2001
ISSN: 1424-8581
Statement of
F. Grützner, E. Zend-Ajusch, K. Stout, S. Munsche, A. Niveleau, I. Nanda, M. Schmid and T. Haaf
Abstract: Microdissection of single chicken microchromosomes (MICs) followed by degenerate oligonucleotide-primed (DOP) PCR allows the rapid generation of MIC-specific DNA libraries. Since some libraries derived from a single (or a few) chromosome(s) label the entire MIC fraction, the majority of chicken MICs share repetitive DNA sequences that are not found on the macrochromosomes. In evolutionarily distant bird species, MICs are invariably hypermethylated. Methylcytosine staining provides additional in situ evidence for the high gene content of MICs and strong compartmentalization of avian genomes.
Keywords: Chromosomes
DNA Probes
Chromosome Painting
Sensitivity and Specificity
Polymerase Chain Reaction
Evolution, Molecular
DNA Methylation
GC Rich Sequence
Repetitive Sequences, Nucleic Acid
Substrate Specificity
Gene Library
Description: Copyright © 2001 S. Karger AG
DOI: 10.1159/000056996
Published version:
Appears in Collections:Aurora harvest
Environment Institute Leaders publications
Molecular and Biomedical Science publications

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