Adelaide Research & Scholarship
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https://hdl.handle.net/2440/34771
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| Type: | Journal article |
| Title: | Chicken microchromosomes are hypermethylated and can be identified by specific painting probes |
| Author: | Grutzner, F. Tsend-Ayush, E. Stout, K. Munsche, S. Niveleau, A. Nanda, I. Schmid, M. Haaf, T. |
| Citation: | Cytogenetic and Genome Research, 2001; 93(3-4):265-269 |
| Publisher: | Karger |
| Issue Date: | 2001 |
| ISSN: | 1424-8581 1424-859X |
| Statement of Responsibility: | F. Grützner, E. Zend-Ajusch, K. Stout, S. Munsche, A. Niveleau, I. Nanda, M. Schmid and T. Haaf |
| Abstract: | Microdissection of single chicken microchromosomes (MICs) followed by degenerate oligonucleotide-primed (DOP) PCR allows the rapid generation of MIC-specific DNA libraries. Since some libraries derived from a single (or a few) chromosome(s) label the entire MIC fraction, the majority of chicken MICs share repetitive DNA sequences that are not found on the macrochromosomes. In evolutionarily distant bird species, MICs are invariably hypermethylated. Methylcytosine staining provides additional in situ evidence for the high gene content of MICs and strong compartmentalization of avian genomes. |
| Keywords: | Chromosomes Animals Chickens Palaeognathae DNA Probes Chromosome Painting Sensitivity and Specificity Polymerase Chain Reaction Evolution, Molecular DNA Methylation GC Rich Sequence Repetitive Sequences, Nucleic Acid Substrate Specificity Gene Library Genome |
| Description: | Copyright © 2001 S. Karger AG |
| DOI: | 10.1159/000056996 |
| Appears in Collections: | Aurora harvest Environment Institute Leaders publications Molecular and Biomedical Science publications |
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