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Type: Journal article
Title: Chicken microchromosomes are hypermethylated and can be identified by specific painting probes
Author: Grutzner, F.
Tsend-Ayush, E.
Stout, K.
Munsche, S.
Niveleau, A.
Nanda, I.
Schmid, M.
Haaf, T.
Citation: Cytogenetic and Genome Research, 2001; 93(3-4):265-269
Publisher: Karger
Issue Date: 2001
ISSN: 1424-8581
Statement of
F. Grützner, E. Zend-Ajusch, K. Stout, S. Munsche, A. Niveleau, I. Nanda, M. Schmid and T. Haaf
Abstract: Microdissection of single chicken microchromosomes (MICs) followed by degenerate oligonucleotide-primed (DOP) PCR allows the rapid generation of MIC-specific DNA libraries. Since some libraries derived from a single (or a few) chromosome(s) label the entire MIC fraction, the majority of chicken MICs share repetitive DNA sequences that are not found on the macrochromosomes. In evolutionarily distant bird species, MICs are invariably hypermethylated. Methylcytosine staining provides additional in situ evidence for the high gene content of MICs and strong compartmentalization of avian genomes.
Keywords: Chromosomes; Animals; Chickens; Palaeognathae; DNA Probes; Chromosome Painting; Sensitivity and Specificity; Polymerase Chain Reaction; Evolution, Molecular; DNA Methylation; GC Rich Sequence; Repetitive Sequences, Nucleic Acid; Substrate Specificity; Gene Library; Genome
Description: Copyright © 2001 S. Karger AG
RMID: 0020063398
DOI: 10.1159/000056996
Appears in Collections:Molecular and Biomedical Science publications
Environment Institute Leaders publications

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