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https://hdl.handle.net/2440/35764
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dc.contributor.author | Kakavanos, R. | - |
dc.contributor.author | Lehn, P. | - |
dc.contributor.author | Callebaut, I. | - |
dc.contributor.author | Meikle, P. | - |
dc.contributor.author | Parkinson-Lawrence, E. | - |
dc.contributor.author | Hopwood, J. | - |
dc.contributor.author | Brooks, D. | - |
dc.date.issued | 2006 | - |
dc.identifier.citation | FEBS Letters, 2006; 580(1):87-92 | - |
dc.identifier.issn | 0014-5793 | - |
dc.identifier.issn | 1873-3468 | - |
dc.identifier.uri | http://hdl.handle.net/2440/35764 | - |
dc.description | Copyright © 2005 Federation of European Biochemical Societies Published by Elsevier B.V. | - |
dc.description.abstract | Enzyme replacement therapy (ERT) has proven to be an effective therapy for some lysosomal storage disorder (LSD) patients. A potential complication during ERT is the generation of an immune response against the replacement protein. We have investigated the antigenicity of two distantly related glycosidases, alpha-glucosidase (Pompe disease or glycogen storage disease type II, GSD II), and alpha-L-iduronidase (Hurler syndrome, mucopolysaccharidosis type I, MPS I). The linear sequence epitope reactivity of affinity purified polyclonal antibodies to recombinant human alpha-glucosidase and alpha-L-iduronidase was defined, to both glycosidases. The polyclonal antibodies exhibited some cross-reactive epitopes on the two proteins. Moreover, a monoclonal antibody to the active site of alpha-glucosidase showed cross-reactivity with a catalytic structural element of alpha-L-iduronidase. In a previous study, in MPS I patients who developed an immune response to ERT, this same site on alpha-L-iduronidase was highly antigenic and the last to tolerise following repeated enzyme infusions. We conclude that glycosidases can exhibit cross-reactive epitopes, and infer that this may relate to common structural elements associated with their active sites. | - |
dc.description.statementofresponsibility | Revecca Kakavanos, Pierre Lehn, Isabelle Callebaut, Peter J. Meikle, Emma J. Parkinson-Lawrence, John J. Hopwood and Doug A. Brooks | - |
dc.description.uri | http://www.elsevier.com/wps/find/journaldescription.cws_home/506085/description#description | - |
dc.language.iso | en | - |
dc.publisher | Elsevier Science BV | - |
dc.source.uri | http://dx.doi.org/10.1016/j.febslet.2005.11.053 | - |
dc.subject | Animals | - |
dc.subject | Humans | - |
dc.subject | Mice | - |
dc.subject | Glycogen Storage Disease Type II | - |
dc.subject | Mucopolysaccharidosis I | - |
dc.subject | Lysosomal Storage Diseases | - |
dc.subject | alpha-Glucosidases | - |
dc.subject | Iduronidase | - |
dc.subject | Epitopes | - |
dc.subject | Enzyme-Linked Immunosorbent Assay | - |
dc.subject | Epitope Mapping | - |
dc.title | Common antigenicity for two glycosidases | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1016/j.febslet.2005.11.053 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Brooks, D. [0000-0001-9098-3626] | - |
Appears in Collections: | Aurora harvest 6 Paediatrics publications |
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