Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/37011
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Type: Journal article
Title: Characterization of genetic miscoding lesions caused by postmortem damage
Author: Gilbert, M.
Hansen, A.
Willerslev, E.
Rudbeck, E.
Barnes, I.
Lynnerup, N.
Cooper, A.
Citation: American Journal of Human Genetics, 2003; 72(1):48-61
Publisher: Univ Chicago Press
Issue Date: 2003
ISSN: 0002-9297
1537-6605
Statement of
Responsibility: 
M. Thomas P. Gilbert, Anders J. Hansen, Eske Willerslev, Lars Rudbeck, Ian Barnes, Niels Lynnerup, and Alan Cooper
Abstract: The spectrum of postmortem damage in mitochondrial DNA was analyzed in a large data set of cloned sequences from ancient human specimens. The most common forms of damage observed are two complementary groups of transitions, termed "type 1" (adenine-->guanine/thymine-->cytosine) and "type 2" (cytosine-->thymine/guanine-->adenine). Single-primer extension PCR and enzymatic digestion with uracil-N-glycosylase confirm that each of these groups of transitions result from a single event, the deamination of adenine to hypoxanthine, and cytosine to uracil, respectively. The predominant form of transition-manifested damage varies by sample, though a marked bias toward type 2 is observed with increasing amounts of damage. The two transition types can be used to identify the original strand, light (L) or heavy (H), on which the initial damage event occurred, and this can increase the number of detected jumping-PCR artifacts by up to 80%. No bias toward H-strand-specific damage events is noted within the hypervariable 1 region of human mitochondria, suggesting the rapid postmortem degradation of the secondary displacement (D-loop) H strand. The data also indicate that, as damage increases within a sample, fewer H strands retain the ability to act as templates for enzymatic amplification. Last, a significant correlation between archaeological site and sample-specific level of DNA damage was detected.
Keywords: Humans; DNA Damage; Postmortem Changes; DNA, Mitochondrial; Artifacts; Polymerase Chain Reaction; Bias (Epidemiology); Base Sequence; Time Factors; Archaeology
Description: © 2003 by The American Society of Human Genetics. All rights reserved.
RMID: 0020065933
DOI: 10.1086/345379
Appears in Collections:Earth and Environmental Sciences publications
Australian Centre for Ancient DNA publications
Environment Institute Leaders publications

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