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|Title:||Modulating effect of serum on the stimulation of plasminogen activator inhibitor 2 production in human gingival fibroblasts by lipopolysaccharide and interleukin-1beta|
|Citation:||Journal of the International Academy of Periodontology, 2004; 6(3):81-88|
|Publisher:||FDI World Dental Press Ltd.|
|Y. Xiao and P.M. Bartold|
|Abstract:||<h4>Objective</h4>Plasminogen activator inhibitor-2 (PAI-2) is an important counter proteolysis factor which helps protect tissues from inflammatory stress. The expression of PAI-2 can be modulated by various inflammatory stimulants and mediators. The aim of the present study was to investigate how serum factors, might modulate the effects of lipopolysaccharide (LPS) and interleukin-1beta on PAI-2 production by human gingival fibroblasts.<h4>Methods</h4>Human gingival fibroblasts were exposed to LPS derived from Actinobacillus actinomycetemcomitans or Escherichia coli and a commercial source of interleukin-1beta (IL-1beta). The expression of PAI-2 and its mRNA was monitored by Western blotting, RT-PCR, and Northern blotting.<h4>Results</h4>The results showed that the distribution of PAI-2 synthesised by human gingival fibroblasts (HGF) was mostly as an intracellular protein (47kDa). The presence of serum in the culture media was absolutely necessary for both the secretion of PAI-2 and for the effect of the inflammatory mediators (LPS and IL-1beta). A pattern of PAI-2 response in HGF after LPS stimulation was detected with a quick up-regulation of the PAI-2 mRNA, which was down regulated when extracellular PAI-2 (60kDa mass) levels reached plateau levels. The synthesis of PAI-2 by HGF, in terms of mRNA expression and protein synthesis, was more sensitive to stimulation with lower concentrations of LPS (10 ng/ml) than with higher LPS concentrations.<h4>Conclusions</h4>PAI-2 is a serum dependent molecule with major cytosolic localisation in HGF. Its cellular accumulation and secretion can be modulated by bacterial LPS and IL-1beta through serum factors.|
|Keywords:||Cells, Cultured; Fibroblasts; Fetal Blood; Gingiva; Animals; Cattle; Humans; Lipopolysaccharides; Plasminogen Activator Inhibitor 2; Interleukin-1; Culture Media, Conditioned; Blotting, Western; Blotting, Northern; Analysis of Variance; Reverse Transcriptase Polymerase Chain Reaction; Gene Expression Regulation; Up-Regulation; Dose-Response Relationship, Drug|
|Description:||Copyright © 2004 Journal of the International Academy of Periodontology|
|Appears in Collections:||Dentistry publications|
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