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https://hdl.handle.net/2440/42950
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Type: | Journal article |
Title: | Cupiennin 1a, an antimicrobial peptide from the venom of the neotropical wandering spider Cupiennius salei, also inhibits the formation of nitric oxide by neuronal nitric oxide synthase |
Author: | Pukala, T. Doyle, J. Llewellyn, L. Kuhn-Nentwig, L. Sorrell, M. Separovic, F. Bowie, J. |
Citation: | The Federation of European Biochemical Societies (FEBS) Journal, 2007; 274(7):1778-1784 |
Publisher: | Blackwell Publishing Ltd |
Issue Date: | 2007 |
ISSN: | 1742-464X 1742-4658 |
Statement of Responsibility: | Tara L. Pukala, Jason R. Doyle, Lyndon E. Llewellyn, Lucia Kuhn-Nentwig, Margit A. Apponyi, Frances Separovic and John H. Bowie |
Abstract: | Cupiennin 1a (GFGALFKFLAKKVAKTVAKQAAKQGAKYVVNKQME-NH2) is a potent venom component of the spider Cupiennius salei. Cupiennin 1a shows multifaceted activity. In addition to known antimicrobial and cytolytic properties, cupiennin 1a inhibits the formation of nitric oxide by neuronal nitric oxide synthase at an IC50 concentration of 1.3 ± 0.3 µm. This is the first report of neuronal nitric oxide synthase inhibition by a component of a spider venom. The mechanism by which cupiennin 1a inhibits neuronal nitric oxide synthase involves complexation with the regulatory protein calcium calmodulin. This is demonstrated by chemical shift changes that occur in the heteronuclear single quantum coherence spectrum of 15N-labelled calcium calmodulin upon addition of cupiennin 1a. The NMR data indicate strong binding within a complex of 1 : 1 stoichiometry. |
Keywords: | Animals Spiders Calcium Nitric Oxide Peptides Antimicrobial Cationic Peptides Calmodulin Spider Venoms Enzyme Inhibitors Magnetic Resonance Spectroscopy Amino Acid Sequence Protein Binding Models, Molecular Molecular Sequence Data Nitric Oxide Synthase Type I |
Description: | The definitive version is available at www.blackwell-synergy.com |
DOI: | 10.1111/j.1742-4658.2007.05726.x |
Published version: | http://dx.doi.org/10.1111/j.1742-4658.2007.05726.x |
Appears in Collections: | Aurora harvest 6 Chemistry and Physics publications |
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