Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/43573
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Type: Journal article
Title: Transforming growth factor-β1 null mutation causes infertility in male mice associated with testosterone deficiency and sexual dysfunction
Other Titles: Transforming growth factor-beta1 null mutation causes infertility in male mice associated with testosterone deficiency and sexual dysfunction
Author: Ingram, W.
Robertson, S.
Citation: Endocrinology, 2007; 148(8):4032-4043
Publisher: Endocrine Soc
Issue Date: 2007
ISSN: 0013-7227
0013-7227
Statement of
Responsibility: 
Wendy V. Ingman and Sarah A. Robertson
Abstract: TGFß1 is a multifunctional cytokine implicated in gonad and secondary sex organ development, steroidogenesis, and spermatogenesis. To determine the physiological requirement for TGFß1 in male reproduction, Tgfb1 null mutant mice on a Prkdcscid immunodeficient background were studied. TGFß1-deficient males did not deposit sperm or induce pseudopregnancy in females, despite an intact reproductive tract with morphologically normal penis, seminal vesicles, and testes. Serum and intratesticular testosterone and serum androstenedione were severely diminished in TGFß1-deficient males. Testosterone deficiency was secondary to disrupted pituitary gonadotropin secretion because serum LH and to a lesser extent serum FSH were reduced, and exogenous LH replacement with human chorionic gonadotropin (hCG) induced serum testosterone to control levels. In the majority of TGFß1-deficient males, spermatogenesis was normal and sperm were developmentally competent as assessed by in vitro fertilization. Analysis of sexual behavior revealed that although TGFß1 null males showed avid interest in females and engaged in mounting activity, intromission was infrequent and brief, and ejaculation was not attained. Administration of testosterone to adult males, even after neonatal androgenization, was ineffective in restoring sexual function; however, erectile reflexes and ejaculation could be induced by electrical stimulation. These studies demonstrate the profound effect of genetic deficiency in TGFß1 on male fertility, implicating this cytokine in essential roles in the hypothalamic-pituitary-gonadal axis and in testosterone-independent regulation of mating competence.
Keywords: Testis; Animals; Mice, Inbred C3H; Mice; Mice, Mutant Strains; Infertility, Male; Androstenedione; Testosterone; Estradiol; Androgens; Electric Stimulation; Ejaculation; Penile Erection; Pregnancy; Mutation; Female; Male; Transforming Growth Factor beta1; Sexual Behavior, Animal
Description: Copyright © 2007 by The Endocrine Society
RMID: 0020072478
DOI: 10.1210/en.2006-1759
Appears in Collections:Obstetrics and Gynaecology publications

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