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https://hdl.handle.net/2440/44203
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Type: | Journal article |
Title: | Deficiency of invariant natural killer T cells in coeliac disease |
Author: | Grose, R. Cummins, A. Thompson, F. |
Citation: | Gut, 2007; 56(6):790-795 |
Publisher: | British Med Journal Publ Group |
Issue Date: | 2007 |
ISSN: | 0017-5749 1468-3288 |
Statement of Responsibility: | R H Grose, A G Cummins and F M Thompson |
Abstract: | <h4>Background</h4>Immunoregulatory invariant natural killer (iNK) T cells rapidly produce interleukin (IL)-4 and other cytokines that suppress a Th1 response and are deficient in some autoimmune diseases.<h4>Aim</h4>The aim of this study was to investigate any deficiency of iNK T cells in coeliac disease.<h4>Methods</h4>Blood was collected from 86 subjects with coeliac disease and from 152 healthy control subjects for investigation of Valpha24+ T cells by flow cytometry. iNK T cells were assessed by Valpha24 and alpha-galactosylceramide/CD1d tetramer markers in 23 normal controls and 13 subjects with coeliac disease. Intracellular IL-4 was measured after anti-CD3 antibody stimulation. Duodenal biopsies were obtained in a subgroup of subjects with coeliac disease and control subjects for Valpha24 mRNA expression using relative PCR and for Valpha24+ T cells by immunofluorescence.<h4>Results</h4>The mean numbers of circulating Valpha24+ T cells and iNK T cells in coeliac disease were 27% (p<0.001) and 16% (p<0.001), respectively, of levels in control subjects. After in vitro anti-CD3 stimulation, numbers of IL-4+ producing iNK T cells from subjects with coeliac disease were unchanged but increased by 21% in control subjects. In subjects with coeliac disease, Valpha24 mRNA intestinal expression was reduced to 17% (p<0.001) by relative PCR and numbers of intestinal Valpha24+ T cells were 16% (p<0.01) of levels in control subjects.<h4>Conclusions</h4>We conclude that Valpha24+ T cells and iNK T cells are deficient in coeliac disease. We speculate that this deficiency could contribute to the failure of immunological oral tolerance that seems to underlie this disease. |
Keywords: | Duodenum Killer Cells, Natural T-Lymphocyte Subsets Cells, Cultured Humans Celiac Disease RNA, Messenger Antigens, Differentiation, B-Lymphocyte Interleukin-4 Histocompatibility Antigens Class II Lymphocyte Count Gene Expression Adult Aged Middle Aged |
Description: | Copyright © 2007 BMJ Publishing Group Ltd & British Society of Gastroenterology |
Provenance: | Published Online First: 24 November 2006. |
DOI: | 10.1136/gut.2006.095307 |
Published version: | http://dx.doi.org/10.1136/gut.2006.095307 |
Appears in Collections: | Aurora harvest 6 Medicine publications |
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