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https://hdl.handle.net/2440/44387
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Type: | Journal article |
Title: | Increased intracellular T helper 1 proinflammatory cytokine production in peripheral blood, bronchoalveolar lavage and intraepithelial T cells of COPD subjects |
Author: | Hodge, G. Nairn, J. Holmes, M. Reynolds, P. Hodge, S. |
Citation: | Clinical and Experimental Immunology, 2007; 150(1):22-29 |
Publisher: | Blackwell Publishing Ltd |
Issue Date: | 2007 |
ISSN: | 0009-9104 1365-2249 |
Statement of Responsibility: | G. Hodge, J. Nairn, M. Holmes, P. N. Reynolds and S. Hodge |
Abstract: | The role of T cells in the pathophysiology of chronic obstructive pulmonary disease (COPD) is not yet certain, although varying reports have shown increases in T helper 1 (Th1) and/or Th2 cytokines in peripheral blood and bronchoalveolar lavage (BAL). No studies have examined cytokine production by intraepithelial T cells obtained by bronchial brushing (BB). Intracellular cytokine analysis of T cell subsets from peripheral blood, BAL and BB from smoker and ex-smoker COPD patients, COPD patients receiving inhaled corticosteroids and smoker and non-smoker control subjects was studied using multi-parameter flow cytometry. CD4 : CD8 inversion was noted in the peripheral blood of smoker and ex-smoker COPD groups, in BAL and BB from smoker controls and BAL of COPD smokers. There was an increase in intracellular CD8(+) T cell Th1 proinflammatory cytokines in some COPD groups in the peripheral blood and in CD8(+) T cell tumour necrosis factor (TNF)-alpha in some COPD groups and smoker controls in BAL and BB. There was an increase in proinflammatory cytokines in COPD smokers compared with ex-smokers and a decrease in COPD smokers receiving inhaled corticosteroids in the airways. There was a negative correlation between forced expiratory volume in 1 s (FEV(1)) and the percentage of BAL and intraepithelial CD8(+) T cells producing TNF-alpha. COPD patients exhibit systemic inflammation as evidenced by increased intracellular Th1 proinflammatory cytokines in blood, BAL and intraepithelial CD8(+) T cells, whereas smoker controls showed localized Th1 response in the lung only. Systemic therapeutic targeting of TNF-alpha production by CD8(+) T cells may improve morbidity in COPD patients while targeting of TNF-alpha in the lung may prevent smokers progressing to COPD. |
Keywords: | Bronchi Respiratory Mucosa Th1 Cells CD8-Positive T-Lymphocytes Bronchoalveolar Lavage Fluid Humans Pulmonary Disease, Chronic Obstructive Inflammation Mediators Cytokines Bronchoscopy Forced Expiratory Volume Lymphocyte Count CD4 Lymphocyte Count Adult Aged Middle Aged Female Male |
Description: | The definitive version is available at www.blackwell-synergy.com |
DOI: | 10.1111/j.1365-2249.2007.03451.x |
Published version: | http://dx.doi.org/10.1111/j.1365-2249.2007.03451.x |
Appears in Collections: | Aurora harvest 6 Paediatrics publications |
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