Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/45409
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dc.contributor.authorShi, B.-
dc.contributor.authorSymons, R.-
dc.contributor.authorPalukaitis, P.-
dc.date.issued2008-
dc.identifier.citationNucleic Acids Research, 2008; 36(4):1057-1071-
dc.identifier.issn0305-1048-
dc.identifier.issn1362-4962-
dc.identifier.urihttp://hdl.handle.net/2440/45409-
dc.descriptionPublished by OUP.-
dc.description.abstractRNA–RNA recombination is an important pathway in virus evolution and has been described for many viruses. However, the factors driving recombination or promoting the selection of recombinants are still unclear. Here, we show that the small movement protein (2b) was able to promote selection of RNA 1/2–RNA 3 recombinants within a chimeric virus having RNAs 1 and 2 from cucumber mosaic virus, and RNA 3 from the related tomato aspermy virus, along with heterologous 2b genes. The source of the 2b also determined the selection of the acceptor RNA and the crossover site, as well as affecting the rate of selection of the recombinant RNAs. The nature of the RNA 3 also influenced the selection of the recombinant RNAs. A 163-nt tandem repeat in RNA 3 significantly affected the rate of selection of the recombinant RNA, while a single nucleotide within the repeat affected the crossover site. The recombination occurred in a non-random manner, involved no intermediates and probably was generated via a copy-choice mechanism during (+) strand RNA synthesis.-
dc.description.statementofresponsibilityBu-Jun Shi, Robert H. Symons and Peter Palukaitis-
dc.language.isoen-
dc.publisherOxford University Press-
dc.rightsCopyright © 2007 The Author(s) This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.-
dc.subjectCucumovirus-
dc.subjectViral Proteins-
dc.subjectRNA, Viral-
dc.subjectRecombination, Genetic-
dc.subjectCrossing Over, Genetic-
dc.subjectBase Sequence-
dc.subjectTandem Repeat Sequences-
dc.subjectConserved Sequence-
dc.subjectNucleic Acid Conformation-
dc.subjectGenome, Viral-
dc.subjectModels, Genetic-
dc.subjectMolecular Sequence Data-
dc.titleThe cucumovirus 2b gene drives selection of inter-viral recombinants affecting the crossover site the acceptor RNA and the rate of selection-
dc.typeJournal article-
dc.provenanceNucleic Acids Research Advance Access originally published online on December 17, 2007-
dc.identifier.doi10.1093/nar/gkm1036-
pubs.publication-statusPublished-
Appears in Collections:Agriculture, Food and Wine publications
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