Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/46231
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Type: Journal article
Title: Morphological characterisation and genetic analysis of a bi-pistil mutant ( bip ) in Medicago truncatula Gaertn.
Author: Nair, R.
Peck, D.
Dundas, I.
Samac, D.
Moore, A.
Randles, J.
Citation: Sexual Plant Reproduction, 2008; 21(2):133-141
Publisher: Springer
Issue Date: 2008
ISSN: 0934-0882
1432-2145
Statement of
Responsibility: 
Ramakrishnan M. Nair, David M. Peck, Ian S. Dundas, Deborah A. Samac, Adam Moore and John W. Randle
Abstract: A floral organ mutant was observed in transgenic Medicago truncatula Gaertn. plants that had two separate stigmas borne on two separate styles that emerged from a single superior carpel primordium. We propose the name bi-pistil, bip for the mutation. We believe this is the first report of such a mutation in this species. Genetic and molecular analyses of the mutant were conducted. The mutant plant was crossed to a mtapetala plant with a wild-type pistil. Expression of the mutant trait in the F1 and F2 generations indicates that the bi-pistil trait is under the control of a single recessive gene. Other modifying genes may influence its expression. The mutation was associated with the presence of a T-DNA insert consisting of the Alfalfa mosaic virus (AMV) coat protein gene in antisense orientation and the nptII selectable marker gene. It is suggested that the mutation is due to gene disruption because multiple copies of the T-DNA were observed in the mutant. The bi-pistil gene was found to be independent of the male-sterile gene, tap. This novel mutant may assist in understanding pistil development in legumes.
Keywords: Medicago truncatula; Legume; Mutant; Floral organ; Bi-pistil
Description: Published online: 12 March 2008 The original publication can be found at www.springerlink.com
RMID: 0020080778
DOI: 10.1007/s00497-008-0073-0
Appears in Collections:Agriculture, Food and Wine publications

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