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|Title:||Left ventricular aneurysm repair in rats: Structural, functional, and molecular consequences|
|Citation:||Journal of Thoracic and Cardiovascular Surgery, 2001; 121(4):750-761|
|Genichi Sakaguchi, Richard L. Young, Masashi Komeda, Kazou Yamanaka, Brian F. Buxton, William J. Louis|
|Abstract:||<h4>Objectives</h4>This study examined the effects of aneurysm repair in a rat model of myocardial infarction on functional indices and on the spatiotemporal distribution of cardiac contractile protein and natriuretic peptide messenger RNA.<h4>Methods</h4>In a rat infarct model, expanded left ventricular aneurysms were plicated 4 weeks after infarction. At 30 weeks, transverse heart sections were taken at 4 levels (apex [level 1] through base [level 4]) and assessed by in situ hybridization histochemistry to determine regional messenger RNA levels of pre-pro-atrial natriuretic peptide, cardiac alpha-actin, skeletal alpha-actin, myosin light chain-2v, and beta-myosin heavy chain.<h4>Results</h4>Rats with plicated left ventricular aneurysms had reduced left ventricular endocardial circumference (19%, P <.005), lower heart weight ratio (31%, P <.05), left ventricular end-diastolic pressures (51%, P <.05), and increased +/-dP/dt (34%-38%, P <.05). Cardiac messenger RNA levels of pre-pro-atrial natriuretic peptide were reduced in the septum (levels 2 and 3), and skeletal alpha-actin levels were reduced in the septum and left ventricular free wall of plicated rats (level 3). beta-Myosin heavy chain levels were markedly reduced in peri-infarct regions of the left ventricular free wall, septum, and right ventricle in plicated rats at level 4, whereas myosin light chain-2v levels were reduced at levels 2 and 4 in the left ventricular free wall and at level 4 in the right ventricle.<h4>Conclusions</h4>Plication of left ventricular aneurysm after infarction in the rat significantly reduced cardiac hypertrophy, improved cardiac function, and reduced the upregulation of pre-pro-atrial natriuretic peptide and both fetal and adult contractile protein isoforms associated with cardiac hypertrophy.|
|Keywords:||Myocardium; Heart Septum; Heart Ventricles; Animals; Rats; Rats, Wistar; Heart Aneurysm; Actins; Atrial Natriuretic Factor; Cardiac Myosins; Myosin Light Chains; Myosin Heavy Chains; Protein Precursors; DNA Probes; RNA, Messenger; Organ Size; In Situ Hybridization; Ventricular Function, Left; Female; Biomarkers|
|Description:||Copyright © 2001 The American Association for Thoracic Surgery|
|Appears in Collections:||Medicine publications|
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