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Type: Journal article
Title: Left ventricular aneurysm repair in rats: Structural, functional, and molecular consequences
Author: Sakaguchi, G.
Young, R.
Komeda, M.
Yamanaka, K.
Buxton, B.
Louis, W.
Citation: Journal of Thoracic and Cardiovascular Surgery, 2001; 121(4):750-761
Publisher: Mosby Inc
Issue Date: 2001
ISSN: 0022-5223
Statement of
Genichi Sakaguchi, Richard L. Young, Masashi Komeda, Kazou Yamanaka, Brian F. Buxton, William J. Louis
Abstract: <h4>Objectives</h4>This study examined the effects of aneurysm repair in a rat model of myocardial infarction on functional indices and on the spatiotemporal distribution of cardiac contractile protein and natriuretic peptide messenger RNA.<h4>Methods</h4>In a rat infarct model, expanded left ventricular aneurysms were plicated 4 weeks after infarction. At 30 weeks, transverse heart sections were taken at 4 levels (apex [level 1] through base [level 4]) and assessed by in situ hybridization histochemistry to determine regional messenger RNA levels of pre-pro-atrial natriuretic peptide, cardiac alpha-actin, skeletal alpha-actin, myosin light chain-2v, and beta-myosin heavy chain.<h4>Results</h4>Rats with plicated left ventricular aneurysms had reduced left ventricular endocardial circumference (19%, P <.005), lower heart weight ratio (31%, P <.05), left ventricular end-diastolic pressures (51%, P <.05), and increased +/-dP/dt (34%-38%, P <.05). Cardiac messenger RNA levels of pre-pro-atrial natriuretic peptide were reduced in the septum (levels 2 and 3), and skeletal alpha-actin levels were reduced in the septum and left ventricular free wall of plicated rats (level 3). beta-Myosin heavy chain levels were markedly reduced in peri-infarct regions of the left ventricular free wall, septum, and right ventricle in plicated rats at level 4, whereas myosin light chain-2v levels were reduced at levels 2 and 4 in the left ventricular free wall and at level 4 in the right ventricle.<h4>Conclusions</h4>Plication of left ventricular aneurysm after infarction in the rat significantly reduced cardiac hypertrophy, improved cardiac function, and reduced the upregulation of pre-pro-atrial natriuretic peptide and both fetal and adult contractile protein isoforms associated with cardiac hypertrophy.
Keywords: Myocardium
Heart Septum
Heart Ventricles
Rats, Wistar
Heart Aneurysm
Atrial Natriuretic Factor
Cardiac Myosins
Myosin Light Chains
Myosin Heavy Chains
Protein Precursors
DNA Probes
RNA, Messenger
Organ Size
In Situ Hybridization
Ventricular Function, Left
Description: Copyright © 2001 The American Association for Thoracic Surgery
DOI: 10.1067/mtc.2001.112462
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