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https://hdl.handle.net/2440/50661
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dc.contributor.author | Appelbee, M. | - |
dc.contributor.author | Mekuria, G. | - |
dc.contributor.author | Nagasandra, V. | - |
dc.contributor.author | Bonneau, J. | - |
dc.contributor.author | Eagles, H. | - |
dc.contributor.author | Eastwood, R. | - |
dc.contributor.author | Mather, D. | - |
dc.date.issued | 2009 | - |
dc.identifier.citation | Journal of Cereal Science, 2009; 49(2):254-261 | - |
dc.identifier.issn | 0733-5210 | - |
dc.identifier.issn | 1095-9963 | - |
dc.identifier.uri | http://hdl.handle.net/2440/50661 | - |
dc.description.abstract | Low-molecular weight glutenin subunits (LWM-GS) are important components of wheat (Triticum aestivum L.) gluten, with important effects on end-use quality. The LMW-GS are encoded at Glu-3 loci (Glu-A3, Glu-B3 and Glu-D3, on the short arms of chromosomes 1A, 1B and 1D), each of which exhibits extensive allelic variation. Each locus encodes numerous LMW-GS, some of which have similar electrophoretic mobilities, making it difficult to distinguish among Glu-3 loci. Alleles of the Glu-D3 locus of bread wheat are considered the most problematic to assign. To date, six Glu-D3 alleles, designated a, b, c, d, e and f, have been reported. We report five previously undescribed alleles (g, h, i, j and k), and describe a method for characterizing them using a combination of SDS-PAGE and multiplexed PCR-based DNA markers. This method could be used for accurate identification of Glu-D3 alleles, permitting the estimation of the effects of these alleles on end-use quality and the selection of desirable alleles and allelic combinations in wheat breeding. | - |
dc.description.statementofresponsibility | M.-J. Appelbee, G.T. Mekuria, V. Nagasandra, J.P. Bonneau, H.A. Eagles, R.F. Eastwood and D.E. Mather | - |
dc.language.iso | en | - |
dc.publisher | Academic Press Ltd Elsevier Science Ltd | - |
dc.rights | Crown copyright © 2008 Published by Elsevier Ltd. | - |
dc.source.uri | http://dx.doi.org/10.1016/j.jcs.2008.10.011 | - |
dc.subject | Glu-3 | - |
dc.subject | Glu-D3 | - |
dc.subject | Low-molecular weight glutenin | - |
dc.subject | Protein | - |
dc.subject | Wheat | - |
dc.title | Novel allelic variants encoded at the Glu-D3 locus in bread wheat | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1016/j.jcs.2008.10.011 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Mather, D. [0000-0001-7506-2589] | - |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest |
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