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|Title:||Single primer extension (SPEX) amplification to accurately genotype highly damaged DNA templates|
|Citation:||Forensic Science International: Genetics Supplement Series, 2008; 1(1):19-21|
|Paul Brotherton, Phillip Endicott, Mark Beaumont, Ross Barnett, Jeremy Austin, Alan Cooper, Juan J. Sanchez|
|Abstract:||High levels of non-authentic sequence data can be generated by traditional PCR-based methodologies when DNA is damaged, template numbers are small and/or the target amplification size too large. We therefore present an alternate methodology based on single primer extension (SPEX) amplification; that places no pre-defined size constraints on amplification and interacts with only one of the DNA strands at the target locus. © 2008 Elsevier Ireland Ltd. All rights reserved.|
Low copy number DNA
|Appears in Collections:||Aurora harvest|
Earth and Environmental Sciences publications
Environment Institute Leaders publications
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